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Listeria monocytogenes

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Kieran Jordan

    1. Teagasc Food Research Centre, Fermoy, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Edward M. Fox

    1. Food Microbiology and Safety Group, Animal, Food and Health Sciences, CSIRO, Werribee, Australia

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    You can also search for this editor in PubMed   Google Scholar

  3. Martin Wagner

    1. University of Veterinary Medicine, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols to study Listeria monocytogenes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1157)

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About this book

Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-x
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  2. Detection, Quantification, and Confirmation

    1. Front Matter

      Pages 1-1
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    2. Sampling the Processing Environment for Listeria

      • Anca Ioana Nicolau, Andrei Sorin Bolocan
      Pages 3-14

    3. Traditional Methods for Isolation of Listeria monocytogenes

      • Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, Paula Teixeira
      Pages 15-30

    4. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR

      • David Rodríguez-Lázaro, Marta Hernández
      Pages 31-38

  3. Characterization and Typing

    1. Front Matter

      Pages 39-39
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    2. Serotype Assignment by Sero-Agglutination, ELISA, and PCR

      • Lisa Gorski
      Pages 41-61

    3. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

      • Marion Dalmasso, Kieran Jordan
      Pages 63-72

    4. Multilocus Sequence Typing (MLST) of Listeria monocytogenes

      • Beatrix Stessl, Irene Rückerl, Martin Wagner
      Pages 73-83

    5. Ribotyping and Automated Ribotyping of Listeria monocytogenes

      • Mazin Matloob, Mansel Griffiths
      Pages 85-93

    6. Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes

      • Corinne Amar
      Pages 95-101

    7. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

      • Edward M. Fox, Kieran Jordan
      Pages 103-108

    8. Analysis of Listeria monocytogenes Subproteomes

      • Michel Hébraud
      Pages 109-128

    9. The Listeria Cell Wall and Associated Carbohydrate Polymers

      • Marcel R. Eugster, Martin J. Loessner
      Pages 129-140

    10. Use of Bacteriophage Cell Wall-Binding Proteins for Rapid Diagnostics of Listeria

      • Mathias Schmelcher, Martin J. Loessner
      Pages 141-156

    11. Virulence Characterization of Listeria monocytogenes

      • Swetha Reddy, Mark L. Lawrence
      Pages 157-165

    12. Internalization Assays for Listeria monocytogenes

      • Andreas Kühbacher, Pascale Cossart, Javier Pizarro-Cerdá
      Pages 167-178

  4. Strain Manipulation

    1. Front Matter

      Pages 179-179
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    2. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes

      • Aidan Casey, Olivia McAuliffe
      Pages 181-186

    3. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method

      • Kathrin Rychli, Caitriona M. Guinane, Karen Daly, Colin Hill, Paul D. Cotter
      Pages 187-200
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Editors and Affiliations

  • Teagasc Food Research Centre, Fermoy, Ireland

    Kieran Jordan

  • Food Microbiology and Safety Group, Animal, Food and Health Sciences, CSIRO, Werribee, Australia

    Edward M. Fox

  • University of Veterinary Medicine, Vienna, Austria

    Martin Wagner

Bibliographic Information

  • Book Title: Listeria monocytogenes

  • Book Subtitle: Methods and Protocols

  • Editors: Kieran Jordan, Edward M. Fox, Martin Wagner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0703-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0702-1Published: 04 May 2014

  • Softcover ISBN: 978-1-4939-5436-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0703-8Published: 03 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 286

  • Number of Illustrations: 25 b/w illustrations, 17 illustrations in colour

  • Topics: Microbiology, Food Science

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