Book 2014

Membrane Proteins Production for Structural Analysis

Editors:

ISBN: 978-1-4939-0661-1 (Print) 978-1-4939-0662-8 (Online)

Table of contents (15 chapters)

  1. Front Matter

    Pages i-xxiii

  2. No Access

    Chapter

    Pages 1-44

    Membrane Protein Production for Structural Analysis

  3. No Access

    Chapter

    Pages 45-70

    Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies

  4. No Access

    Chapter

    Pages 71-86

    Bacterial Expression and Stabilization of GPCRs

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    Chapter

    Pages 87-106

    Membrane Protein Production in Escherichia coli: Overview and Protocols

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    Chapter

    Pages 107-132

    Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

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    Chapter

    Pages 133-171

    Overexpression of Membrane Proteins in Saccharomyces cerevisiae for Structural and Functional Studies: A Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p

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    Chapter

    Pages 173-203

    Amphipols: A General Introduction and Some Protocols

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    Chapter

    Pages 205-251

    New Amphiphiles to Handle Membrane Proteins: “Ménage à Trois” Between Chemistry, Physical Chemistry, and Biochemistry

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    Chapter

    Pages 253-266

    Building Model Membranes with Lipids and Proteins: Dangers and Challenges

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    Chapter

    Pages 267-287

    Analytical Ultracentrifugation and Size-Exclusion Chromatography Coupled with Light Scattering for the Characterization of Membrane Proteins in Solution

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    Chapter

    Pages 289-314

    Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins

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    Chapter

    Pages 315-345

    Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR

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    Chapter

    Pages 347-392

    Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins

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    Chapter

    Pages 393-421

    Structural Studies of TSPO, a Mitochondrial Membrane Protein

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    Chapter

    Pages E1-E1

    Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins

  17. Back Matter

    Pages 423-425