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Yeast Metabolic Engineering

Methods and Protocols

  • Book
  • © 2014


  • Includes cutting-edge methods and protocols for the metabolic engineering of numerous strains of yeast
  • Provides step-by-step detail essential for reproducible results in the lab
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1152)

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About this book

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

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Table of contents (19 protocols)

  1. Molecular Tools and Technology for Yeast Engineering

  2. Tools and Technologies for Investigation and Determination of Yeast Metabolic Features

  3. Metabolic Models for Yeast Metabolic Engineering

Editors and Affiliations

  • Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden

    Valeria Mapelli

Bibliographic Information

  • Book Title: Yeast Metabolic Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Valeria Mapelli

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-4939-0562-1Published: 18 April 2014

  • Softcover ISBN: 978-1-4939-5286-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0563-8Published: 17 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 316

  • Number of Illustrations: 26 b/w illustrations, 18 illustrations in colour

  • Topics: Biotechnology, Gene Expression

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