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  • Book
  • © 2014

Engineering and Analyzing Multicellular Systems

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1151)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-0554-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 139.99
Price excludes VAT (USA)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Constructing Multicellular Systems

    1. Front Matter

      Pages 1-1
    2. Recent Progress in Engineering Human-Associated Microbiomes

      • Stephanie J. Yaung, George M. Church, Harris H. Wang
      Pages 3-25
    3. Transformation of Bacillus subtilis

      • Xiao-Zhou Zhang, Chun You, Yi-Heng Percival Zhang
      Pages 95-101
    4. Culturing Anaerobes to Use as a Model System for Studying the Evolution of Syntrophic Mutualism

      • Sujung Lim, Sergey Stolyar, Kristina Hillesland
      Pages 103-115
    5. Therapeutic Microbes for Infectious Disease

      • Choon Kit Wong, Mui Hua Tan, Bahareh Haji Rasouliha, In Young Hwang, Hua Ling, Chueh Loo Poh et al.
      Pages 117-133
  3. Analyzing and Modeling Multicellular Systems

    1. Front Matter

      Pages 135-135
    2. Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Membrane

      • Kazufumi Hosoda, Naoaki Ono, Shingo Suzuki, Tetsuya Yomo
      Pages 151-164
    3. 3D-Fluorescence In Situ Hybridization of Intact, Anaerobic Biofilm

      • Kristen A. Brileya, Laura B. Camilleri, Matthew W. Fields
      Pages 189-197
    4. Simulating Microbial Community Patterning Using Biocellion

      • Seunghwa Kang, Simon Kahan, Babak Momeni
      Pages 233-253
  4. Back Matter

    Pages 255-256

About this book

Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Engineering Multicellular Systems: Methods and Protocols provides a comprehensive laboratory protocol reference for constructing multicellular systems for various applications.​

Keywords

  • Microbial ecosystems
  • cell-cell interactions
  • engineering genetic
  • microbial communities
  • microbial species
  • predictive mathematical models
  • synthetic communities
  • biochemical engineering

Editors and Affiliations

  • School of Life Sciences, University of Science & Technology of China,, Hefei, China

    Lianhong Sun

  • Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, USA

    Wenying Shou

Bibliographic Information

  • Book Title: Engineering and Analyzing Multicellular Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Lianhong Sun, Wenying Shou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0554-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0553-9

  • Softcover ISBN: 978-1-4939-5272-4

  • eBook ISBN: 978-1-4939-0554-6

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 256

  • Number of Illustrations: 23 b/w illustrations, 86 illustrations in colour

  • Topics: Biotechnology, Chemical Bioengineering

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-0554-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 139.99
Price excludes VAT (USA)