Advertisement

Electroporation and Electrofusion in Cell Biology

  • Eberhard Neumann
  • Arthur E. Sowers
  • Carol A. Jordan

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Cells in Electric Fields

    1. Front Matter
      Pages 1-1
    2. H. P. Schwan
      Pages 3-21
    3. Ingolf Lamprecht, Maja Mischel
      Pages 23-35
    4. Francisco J. Iglesias, Carlos Santamaría, M. Carmen López, Angel Domínguez
      Pages 37-57
  3. Electroporation

    1. Front Matter
      Pages 59-59
    2. Leonid V. Chernomordik, Yuri A. Chizmadzhev
      Pages 83-95
    3. James C. Weaver, Kevin T. Powell
      Pages 111-126
    4. Bernhard Deuticke, Karl Schwister
      Pages 127-148
    5. Tian Yow Tsong
      Pages 149-163
  4. Electrofusion

    1. Front Matter
      Pages 165-165
    2. David A. Stenger, Sek Wen Hui
      Pages 167-180
    3. Grigory B. Melikyan, Leonid V. Chernomordik
      Pages 181-192
    4. Takako Ohno-Shosaku, Yasunobu Okada
      Pages 193-202
    5. J. Teissié, M. P. Rols, C. Blangero
      Pages 203-214
  5. Applications

    1. Front Matter
      Pages 257-257
    2. Mathew M. S. Lo, Tian Yow Tsong
      Pages 259-270
    3. Mark C. Glassy, Michael Pratt
      Pages 271-282
    4. D. E. Knight
      Pages 283-297
    5. Walter Förster, Eberhard Neumann
      Pages 299-318
    6. M. J. O’Hare, M. G. Ormerod, P. R. Imrie, J. H. Peacock, W. Asche
      Pages 319-330
    7. Huntington Potter
      Pages 331-341
    8. James A. Saunders, Benjamin F. Matthews, Paul D. Miller
      Pages 343-354
    9. Barbara Junker, Horst Lörz
      Pages 377-386
  6. Methods and Equipment

    1. Front Matter
      Pages 387-387
    2. Gunter A. Hofmann
      Pages 389-407
  7. Back Matter
    Pages 433-436

About this book

Introduction

Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.

Keywords

Cytosol Expression Lipid Mammalia cell biology fluorescence gene transfer microorganism

Editors and affiliations

  • Eberhard Neumann
    • 1
  • Arthur E. Sowers
    • 2
  • Carol A. Jordan
    • 3
  1. 1.University of BielefeldBielefeldFRG
  2. 2.American Red CrossRockvilleUSA
  3. 3.Science Applications International CorporationMcLeanUSA

Bibliographic information