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Low-Temperature Microscopy and Analysis

  • Patrick Echlin

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Patrick Echlin
    Pages 1-17
  3. Patrick Echlin
    Pages 59-99
  4. Patrick Echlin
    Pages 101-140
  5. Patrick Echlin
    Pages 193-222
  6. Patrick Echlin
    Pages 265-293
  7. Patrick Echlin
    Pages 349-411
  8. Patrick Echlin
    Pages 413-490
  9. Patrick Echlin
    Pages 499-528
  10. Back Matter
    Pages 529-539

About this book

Introduction

The frozen-hydrated specimen is the principal element that unifies the subject of low­ temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com­ mon fact and theory upon which their work must be based. Low-Temperature Micros­ copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light­ optical microscopes.

Keywords

Hydra biological electron electron microscopy illustration low temperature microscopy replication structure temperature transmission electron microscopy water

Authors and affiliations

  • Patrick Echlin
    • 1
  1. 1.University of CambridgeCambridgeEngland

Bibliographic information