Electrochemical Sensors in Immunological Analysis

  • T. T. Ngo

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael Thompson, Joseph S. Tauskela, Ulrich J. Krull
    Pages 1-18
  3. Shuichi Suzuki, Masuo Aizawa
    Pages 47-59
  4. H. Allen O. Hill, Nicholas J. Walton
    Pages 61-75
  5. Sam A. McClintock, William C. Purdy
    Pages 77-85
  6. Matthew J. Doyle, Kenneth R. Wehmeyer, William R. Heineman, H. Brian Halsall
    Pages 87-102
  7. D. Scott Wright, H. Brian Halsall, William R. Heineman
    Pages 117-130
  8. Shia S. Kuan, George G. Guilbault
    Pages 145-165

About this book

Introduction

The development of radioimmunoassay (RIA) by R.S. Yalow and S.A. Berson in 1959 opens up a new avenue in ultra­ sensitive analysis of trace substances in complex biological systems. In recognition of the enormous contributions of RIA to basic research in biology and to routine clinical tests in laboratory medicine, R.S. Yalow, the co-developer of RIA, was awarded, in 1977, the Nobel Prize for Medicine and Physiology. The basic principle of RIA is elegantly simple. It is based on a specific, competitive binding reaction between the analyte and the radio-labeled analog of the analyte for the specific antibody raised to the analyte. The combination of high specificity and affinity of an antibody molecule makes it a very versatile analytical reagent capable of reacting specifically with analytes at a very low concentration in a complex solution such as serum. The sensitivity of RIA is provided by using a radioactive tracer.

Keywords

Antigen enzymes laboratory medicine physiology protein reaction

Editors and affiliations

  • T. T. Ngo
    • 1
  1. 1.University of California, IrvineIrvineUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4899-1974-8
  • Copyright Information Springer-Verlag US 1987
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4899-1976-2
  • Online ISBN 978-1-4899-1974-8
  • About this book