Fluorescence Microscopy and Fluorescent Probes

  • Jan Slavík

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Fluorescence Microscopy and Fluorescent Probes

    1. Hans C. Gerritsen
      Pages 35-46
    2. Jan Slavík
      Pages 57-60
    3. José-Enrique O’Connor
      Pages 61-66
    4. Herbert Schneckenburger, Michael H. Gschwend, Karsten König, Reinhard Sailer, Wolfgang S. L. Strauß
      Pages 71-78
    5. I. V. Eigenbrot, B. Crystall, D. Phillips
      Pages 79-83
  3. Ion-Sensitive Fluorescent Probes

  4. Membrane Potential-Sensitive Fluorescent Probes

About this book

Introduction

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Keywords

enzymes fluorescence image analysis imaging microscopy tissue

Editors and affiliations

  • Jan Slavík
    • 1
  1. 1.Institute of PhysiologyCzech Academy of SciencesPragueCzech Republic

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4899-1866-6
  • Copyright Information Springer-Verlag US 1996
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4899-1868-0
  • Online ISBN 978-1-4899-1866-6
  • About this book