Methods in Protein Structure Analysis

1. Front Matter

   Pages i-xvii

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2. Protein and Peptide Preparation for Microsequence Analysis

   1. Front Matter

      Pages 1-1

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   2. Characterization of Proteins Separated by Gel Electrophoresis at the Primary Structure Level

      • Ruedi Aebersold, Lawrence N. Amankwa, Heinz Nika, David T. Chow, Edward J. Bures, Hamish D. Morrison et al.

      Pages 3-14

   3. New Strategies in High Sensitivity Characterization of Proteins Separated from 1-D or 2-D Gels

      • Kris Gevaert, Marc Rider, Magda Puype, Jozef Van Damme, Stefaan De Boeck, Joël Vandekerckhove

      Pages 15-26

   4. High-Speed Chromatographic Separation of Proteins and Peptides for High Sensitivity Microsequence Analysis

      • Robert L. Moritz, James Eddes, Richard J. Simpson

      Pages 27-38

   5. Pre-Electrophoretic Labelling of Proteins with a Coloured Water Soluble Edman Reagent

      • Keith Ashman

      Pages 39-45

3. N-Terminal Sequence Analysis

   1. Front Matter

      Pages 47-47

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   2. High Sensitivity Analysis of PTH Amino Acids

      • Karen C. Waldron, Michael Carpenter, Ian Ireland, Darren Lewis, Xing Fang Li, Norman J. Dovichi

      Pages 49-55

   3. Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polypeptides

      • Edward J. Bures, Heinz Nika, David T. Chow, Daniel Hess, Hamish D. Morrison, Michael Bartlet-Jones et al.

      Pages 57-68

   4. Identification and Characterization of Glycosylated Phenylthiohydantoin Amino Acids

      • Anthony Pisano, Nicolle H. Packer, John W. Redmond, Keith L. Williams, Andrew A. Gooley

      Pages 69-80

   5. Deacetylation and Internal Cleavage of Polypeptides for N-Terminal Sequence Analysis

      • Madalina T. Gheorghe, Tomas Bergman

      Pages 81-86

4. N-Terminal Sequence Analysis

   1. Front Matter

      Pages 87-87

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   2. A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor

      • K. Takamoto, M. Kamo, K. Satake, A. Tsugita

      Pages 89-107

   3. Sequencing of Proteins from the C-Terminus

      • Victoria L. Boyd, MeriLisa Bozzini, Jindong Zhao, Robert J. DeFranco, Pau-Miau Yuan, G. Marc Loudon et al.

      Pages 109-118

   4. C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System

      • Chad G. Miller, Jerome M. Bailey, David H. Hawke, Sherrell Early, Jacqueline Tso

      Pages 119-129

   5. Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Proline

      • Jerome M. Bailey, Oanh Tu, Gilbert Issai, John E. Shively

      Pages 131-138

5. Mass Spectrometry

   1. Front Matter

      Pages 139-139

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   2. Tandem Mass Spectrometric Characterization of Modified Peptides and Proteins

      • Simon J. Gaskell, Mark S. Bolgar, Kathleen A. Cox

      Pages 141-150

   3. Direct Mass Spectrometric Analyses for Protein Chemistry Studies

      • Scott D. Buckel, Tracy I. Stevenson, Joseph A. Loo

      Pages 151-159

   4. Peptide-Mass Fingerprinting as a Tool for the Rapid Identification and Mapping of Cellular Proteins

      • D. J. C. Pappin, D. Rahman, H. F. Hansen, W. Jeffery, C. W. Sutton

      Pages 161-173

The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysis; however, the emphasis remains on "methods" and on "chemistry. " In fact, this is the only major conference that is devoted to methods. The MPSA conference is truly international, a fact clearly reflected by the composi­ tion of its Scientific Advisory Committee. The Scientific Advisory Committee oversees the scientific direction of the MPSA and elects the chairman of the conference. Members of the committee are elected by active members, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. This is different from, and more detailed than, the special MPSA issue of the Journal of Protein Chemistry in which only a brief description of the talks is given in short papers and abstracts. In the I Oth MPSA, about half the talks are by invited speakers and the remainder were selected from submitted short papers and abstracts.

• Amino acid
• chemistry
• preparation
• protein
• sequence analysis

• Baylor College of Medicine, Houston, USA

  M. Zouhair Atassi

• National Cancer Institute, National Institutes of Health, Bethesda, USA

  Ettore Appella

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