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Principles and Methods in Receptor Binding

  • F. Cattabeni
  • S. Nicosia

Part of the NATO ASI Series book series (NSSA, volume 72)

Table of contents

  1. Front Matter
    Pages i-viii
  2. S. J. Enna
    Pages 13-33
  3. Alexander Levitzki
    Pages 35-39
  4. Giancarlo Pepeu, Ileana Marconcini Pepeu
    Pages 65-77
  5. M. Memo, E. Carboni, H. Uzumaki, S. Govoni, M. O. Carruba, M. Trabucchi et al.
    Pages 93-111
  6. Claus Braestrup, Mogens Nielsen, Tage Honoré
    Pages 113-125
  7. Paolo Marchetti, Gigliola Sica, Clara Natoli, Maria Assunta Spina, Stefano Iacobelli
    Pages 155-159
  8. Paolo Marchetti, Giovanni Scambia, Gigliola Sica, Vittoria Natoli, Stefano Iacobelli
    Pages 161-193
  9. Franco Gambale, Cesare Usai, Mauro Robello, Carla Marchetti
    Pages 215-231
  10. Back Matter
    Pages 255-267

About this book

Introduction

One of the major advances in the understanding of the mechanism of action of hormones, neurotransmitters and drugs had arisen from the hypothesis that the physiological or pharmacological responses are trig­ gered by their interaction with specific cell compo­ nents, termed receptors. However, the presence of receptors has been inferred from indirect data, and only recently has it been possible to study the kinetics of the interaction between drug and receptors directly, through the so called "binding technique. " This NATO-ASI on "Principles and Methods in Receptor Binding" was devoted mainly to the following aspects of the study of receptors: the principles underlying the use of the binding technique; the mathematical models necessary to interpret binding data; the application of binding methods to specific receptors; and, finally, a few selected examples of coupling between receptors and physiological re­ sponses. In the chapters of this book, special interest is devoted to the analysis of the simplest models for the interaction of receptors with their ligands (either hormones or neurotransmitters or drugs). The graphical techniques used to analyze the data from binding exper iments are extensively discussed, together with the statistics that have to be used in binding analysis. Moreover, the basic concepts to analyze binding data using a personal computer are presented. v vi PREFACE The factors which should be considered when setting up a binding assay (such as choice of ligand and incubation conditions, preparation of tissue, termina­ tion of incubation) are discussed.

Editors and affiliations

  • F. Cattabeni
    • 1
  • S. Nicosia
    • 2
  1. 1.Institute of Pharmacology and PharacognosyUniversity of UrbinoUrbinoItaly
  2. 2.Institute of Pharmacology and PharmacognosyUniversity of MilanMilanItaly

Bibliographic information