Hydra: Research Methods

  • Howard M. Lenhoff
Book

Table of contents

  1. Front Matter
    Pages i-xxxi
    PDF

  2. Introduction

    1. Introduction
      Howard M. Lenhoff
      Pages 1-3

  3. Terminology for Morphology and Cell Types

    1. Terminology for Morphology and Cell Types
      Richard D. Campbell, Hans R. Bode
      Pages 5-14

  4. Culture and Handling

    1. Front Matter
      Pages 15-15
      PDF
    2. Collecting Hydra
      Richard D. Campbell
      Pages 17-18
    3. Identifying Hydra Species
      Richard D. Campbell
      Pages 19-28
    4. Water, Culture Solutions, and Buffers
      Howard M. Lenhoff
      Pages 29-34
    5. Visual Monitoring of pH in Solutions with Phenol Red
      M. Rahat, Vanda Reich
      Pages 35-37
    6. Hatching Brine Shrimp Larvae Axenically and/or in a Range of Quantities
      Howard M. Lenhoff
      Pages 39-46
    7. Determining Growth Rates of Groups of Hydra and Budding Rates of Individual Hydra
      Howard M. Lenhoff
      Pages 47-52
    8. Culturing Large Numbers of Hydra
      Howard M. Lenhoff
      Pages 53-62
    9. Turbidimetric and Pipetimetric Measurements of Number of Hydra
      Howard M. Lenhoff
      Pages 63-65
    10. Culturing Hydra of the Same Species but of Different Sizes
      Richard D. Campbell, Joann J. Otto
      Pages 67-69
    11. Culture of Sexually Differentiated Hydra
      Charles L. Rutherford, David Hessinger, Howard M. Lenhoff
      Pages 71-77
    12. Preparing Axenic Hydra
      M. Rahat, Vanda Reich
      Pages 79-83

  5. Histology

    1. Front Matter
      Pages 85-85
      PDF
    2. Preparing Hydra for Transmission Electron Microscopy
      Richard L. Wood
      Pages 87-94
    3. Preparing Hydra for Scanning Electron Microscopy
      Richard L. Wood
      Pages 95-104
    4. Preparing Hydra for Freeze-Fracture and Freeze-Etching
      Richard L. Wood
      Pages 105-115
    5. Whole Mounts for Light Microscopy
      Richard D. Campbell
      Pages 117-120

About this book

Keywords

cell controlling cytology development enzymes histology monitoring morphogenesis organizations physiology population stem cells tissue transmission electron microscopy water

Editors and affiliations

  • Howard M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4757-0596-6
  • Copyright Information Springer-Verlag US 1983
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4757-0598-0
  • Online ISBN 978-1-4757-0596-6
  • About this book