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Hydra: Research Methods

  • Howard M. Lenhoff
Book

Table of contents

  1. Front Matter
    Pages i-xxxi
    PDF

  2. Introduction

    1. Introduction
      Howard M. Lenhoff
      Pages 1-3

  3. Terminology for Morphology and Cell Types

    1. Terminology for Morphology and Cell Types
      Richard D. Campbell, Hans R. Bode
      Pages 5-14

  4. Culture and Handling

    1. Front Matter
      Pages 15-15
      PDF
    2. Collecting Hydra
      Richard D. Campbell
      Pages 17-18
    3. Identifying Hydra Species
      Richard D. Campbell
      Pages 19-28
    4. Water, Culture Solutions, and Buffers
      Howard M. Lenhoff
      Pages 29-34
    5. Visual Monitoring of pH in Solutions with Phenol Red
      M. Rahat, Vanda Reich
      Pages 35-37
    6. Hatching Brine Shrimp Larvae Axenically and/or in a Range of Quantities
      Howard M. Lenhoff
      Pages 39-46
    7. Determining Growth Rates of Groups of Hydra and Budding Rates of Individual Hydra
      Howard M. Lenhoff
      Pages 47-52
    8. Culturing Large Numbers of Hydra
      Howard M. Lenhoff
      Pages 53-62
    9. Turbidimetric and Pipetimetric Measurements of Number of Hydra
      Howard M. Lenhoff
      Pages 63-65
    10. Culturing Hydra of the Same Species but of Different Sizes
      Richard D. Campbell, Joann J. Otto
      Pages 67-69
    11. Culture of Sexually Differentiated Hydra
      Charles L. Rutherford, David Hessinger, Howard M. Lenhoff
      Pages 71-77
    12. Preparing Axenic Hydra
      M. Rahat, Vanda Reich
      Pages 79-83

  5. Histology

    1. Front Matter
      Pages 85-85
      PDF
    2. Preparing Hydra for Transmission Electron Microscopy
      Richard L. Wood
      Pages 87-94
    3. Preparing Hydra for Scanning Electron Microscopy
      Richard L. Wood
      Pages 95-104
    4. Preparing Hydra for Freeze-Fracture and Freeze-Etching
      Richard L. Wood
      Pages 105-115
    5. Whole Mounts for Light Microscopy
      Richard D. Campbell
      Pages 117-120
    6. Preparing Histological Sections for Light Microscopy
      Richard D. Campbell
      Pages 121-130
    7. Vital Staining: Fluorescent and Immunofluorescent, and Review of Nonfluorescent Dyes
      John F. Dunne, C. Lynne Littlefield
      Pages 131-140

  6. Macrophotography

    1. Front Matter
      Pages 141-141
      PDF
    2. Macrophotography
      Richard D. Campbell
      Pages 143-149

  7. Quantitative Cytology

    1. Front Matter
      Pages 151-151
      PDF
    2. Dissociating Hydra Tissue into Single Cells by the Maceration Technique
      Charles N. David
      Pages 153-156
    3. Cell Cycle Analysis of Hydra Cells
      Charles N. David
      Pages 157-163
    4. Mitotic Index
      Richard D. Campbell
      Pages 165-168
    5. Measurement of the Numbers of Nematoblasts, Nematocytes, and Nematocysts
      Hans R. Bode, G. Scott Smith, Patricia M. Bode
      Pages 169-182
    6. Marking Epithelial Cells in Living Hydra with India Ink
      Joann J. Otto, Richard D. Campbell
      Pages 183-186

  8. Techniques Using Isotopes

    1. Front Matter
      Pages 187-187
      PDF
    2. Incorporating [3H]Thymidine into Hydra by Microinjection
      Charles N. David
      Pages 189-191
    3. Labeling with Gaseous 14CO2 or by Feeding on Radioactive Tissues
      Howard M. Lenhoff
      Pages 193-196
    4. Fractionating Small Amounts of Radioactive Tissue
      Howard M. Lenhoff
      Pages 197-203
    5. Rapid Whole-Mount Radioautography
      Howard M. Lenhoff
      Pages 205-207

  9. Isolating Hydra Mutants by Sexual Inbreeding

    1. Front Matter
      Pages 209-209
      PDF
    2. Isolating Hydra Mutants by Sexual Inbreeding
      Tsutomu Sugiyama
      Pages 211-221

  10. Manipulating Tissue Organization

    1. Front Matter
      Pages 223-223
      PDF
    2. Grafting: A Rapid Method for Transplanting Tissue
      Harry K. MacWilliams
      Pages 225-232
    3. Quantitative Interpretation of Transplantation Phenomena
      Harry K. MacWilliams
      Pages 233-249
    4. Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells
      Kristine M. Flick, Hans R. Bode
      Pages 251-259
    5. Culturing Interstitial Stem Cells in Hydra Aggregates
      Charles N. David
      Pages 261-266
    6. Separating Viable Tissue Layers
      Georgia E. Lesh-Laurie
      Pages 267-271
    7. Preparing Ectoderm/Endoderm Chimeras
      Nancy Wanek
      Pages 273-277

  11. Manipulating Cellular Composition in Vivo

    1. Front Matter
      Pages 279-279
      PDF
    2. Eliminating All Nonepithelial Cells Using Colchicine
      Beverly A. Marcum, Richard D. Campbell
      Pages 281-286
    3. Culturing Epithelial Hydra
      Beverly A. Marcum
      Pages 287-290
    4. Reducing Populations of Interstitial Cells and Nematoblasts with Hydroxyurea
      Hans R. Bode
      Pages 291-294
    5. Preparing Hydra viridis with Nerve Cells and No Interstitial Cells, or with Neither of These Cell Types
      Patricia Novak
      Pages 295-297
    6. Eliminating Interstitial Cells with Nitrogen Mustard
      Charles N. David
      Pages 299-302

About this book

Keywords

cell controlling cytology development enzymes histology monitoring morphogenesis organizations physiology population stem cells tissue transmission electron microscopy water

Editors and affiliations

  • Howard M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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