Terminal Transferase in Immunobiology and Leukemia

  • Umberto Bertazzoni
  • F. J. Bollum

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Structures and Properties of Terminal Deoxynucleotidyl Transferase

    1. F. J. Bollum
      Pages 1-11
    2. Lucy M. S. Chang, F. J. Bollum
      Pages 25-35
    3. Martin R. Deibel Jr., Mary Sue Coleman, John J. Hutton
      Pages 37-60
    4. Claude Pénit, Maria-José Gelabert, Catherine Transy, Pierre Rouget
      Pages 61-73
    5. G. Damiani, E. Palla, V. Sgaramella, A. I. Scovassi, U. Bertazzoni
      Pages 75-80
    6. Shonen Yoshida, Sigeo Masaki, Hiromu Nakamura, Toshiteru Morita
      Pages 81-85
    7. Prakash Chandra, Ilhan Demirhan, Uwe Ebener
      Pages 87-100
  3. Development of Immunological Reagents for Detection of Terminal Deoxynucleotidyl Transferase

  4. Use of Terminal Deoxynucleotidyl Transferase as a Marker for Studies on Differentiation of Lymphocytes

    1. G. Janossy, N. Tidman, K. F. Bradstock, A. V. Hoffbrand, F. J. Bollum
      Pages 161-176
    2. N. Sacchi, U. Bertazzoni, D. Breviario, P. Plevani, G. Badaracco, E. Ginelli
      Pages 177-183
    3. Claudio Casoli, Antonio Bonati, Riccardo Starcich
      Pages 185-191
    4. D. Breviario, P. Plevani, N. Sacchi, G. Badaracco, U. Bertazzoni, E. Ginelli
      Pages 193-199

About this book


This book contains the proceedings of a conference devoted to the study of the structure and function of Terminal deoxynucleotidyl Transferase (TdT) and its utilization as biochemical marker in immuno­ biology and leukemia, held in Elba, Italy on May 28-31, 1981. The en­ zyme has been known to nucleic acid biochemists for more than 20 years and has proved to be an excellent tool for making deoxypolymers, label­ ing DNA fragments, and adding homopolymer tails to restriction endonu­ clease fragments from DNA. Since the discovery of its peculiar tissue distribution, normally restricted to the thymus and bone marrow, and of its abnormal occur­ rence in human leukemic lymphoblasts, TdT has become one of the most widely used markers in the study of lymphocyte differentiation and in the classification of hematopoietic neoplasia. The subject seemed most appropriate for a meeting where molecular and cellular biologists, immunologists and hematologists could convene for the first time to discuss both basic research and the clinical aspects of the problem. Among the goals achieved by this workshop was the sharing of in­ formation about the enzymology of TdT, biochemical and immunological methodology and the correlation of TdT with other markers in the diag­ nosis of leukemia. The remarkable accordance of results, presented here by five independent hematological institutions, from analysis of TdT in several thousands of leukemic patients, marks the importance of this enzyme as a diagnostic and prognostic tool in these diseases.


DNA biology enzyme enzymes nucleic acid

Editors and affiliations

  • Umberto Bertazzoni
    • 1
  • F. J. Bollum
    • 2
  1. 1.CNR Institute of Biochemical and Evolutionary GeneticsPaviaItaly
  2. 2.Uniformed Services University of the Health SciencesBethesdaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4684-8929-3
  • Copyright Information Springer-Verlag US 1982
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-8931-6
  • Online ISBN 978-1-4684-8929-3
  • Series Print ISSN 0065-2598
  • About this book