Tissue Culture of Epithelial Cells

  • Mary Taub

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Domes

  3. Electrophysiology and Ion Transport in MDCK Cells

    1. Front Matter
      Pages 23-23
    2. L. González-Mariscal, L. Borboa, R. López-Vancell, G. Beaty, M. Cereijido
      Pages 25-36
    3. S. Fernández-Castelo, J. J. Bolívar, R. López-Vancell, G. Beaty, M. Cereijido
      Pages 37-50
  4. Transport of Neutral Solutes by LLC—PK1 Cells

    1. Front Matter
      Pages 69-69
    2. James M. Mullin, Arnost Kleinzeller
      Pages 71-86
    3. Francisco V. Sepúlveda, Jeremy D. Pearson
      Pages 87-104
    4. Dayton S. Misfeldt, Martin J. Sanders
      Pages 105-124
  5. Membrane Proteins and Hormone Receptors

    1. Front Matter
      Pages 125-125
    2. Kathryn E. Meier, Paul A. Insel
      Pages 145-178
    3. J. Craig Venter, Ursina Schmidt, Barbara Eddy, Giorgio Semenza, Claire M. Fraser
      Pages 179-201
  6. Epithelial Cell Cultures and Hormonally Defined Medium

  7. Back Matter
    Pages 281-288

About this book

Introduction

Epithelial cells are present in many different tissues in the body, and possess a diverse number of functional properties. However, all epithelial cells share some common characteristics. The cells possess a morphological polarity (an-apical and basolateral surface), and are interconnected by tight junctions. The epithelial cells also possess the capacity to transport select solutes across the monolayer. Transport systems localized on either the apical or basolateral surface are respon­ sible for this vectorial transport. Such characteristics of epithelial cells can be examined in the tissue culture situation. This volume discusses the use of cell culture techniques to study these fundamental properties of epithelial cells. Ma­ jor questions concerning epithelia which may be examined in culture are ad­ dressed. The approaches which are taken to answer these questions are described in detail with regards to kidney cell cultures. Similar investigations may be done with epithelial cell cultures derived from other tissues, following the kidney cell culture paradigm.

Keywords

cell cell culture cells kidney physiology tissue transport

Editors and affiliations

  • Mary Taub
    • 1
  1. 1.State University of New York at BuffaloBuffaloUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4684-4814-6
  • Copyright Information Springer-Verlag US 1985
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-4816-0
  • Online ISBN 978-1-4684-4814-6
  • About this book