Techniques in Somatic Cell Genetics

  • Jerry W. Shay

Table of contents

  1. Front Matter
    Pages i-xxvi
  2. W. N. Choy, T. V. Gopalakrishnan, J. W. Littlefield
    Pages 11-21
  3. W. Edward Mercer, Renato Baserga
    Pages 23-34
  4. Thomas H. Norwood, Carol J. Zeigler
    Pages 35-45
  5. George E. Veomett
    Pages 67-79
  6. Jerome M. Eisenstadt, Mary C. Kuhns
    Pages 111-121
  7. Godfrey S. Getz, Kathleen L. Kornafel
    Pages 123-137
  8. Claus-Jens Doersen, Eric J. Stanbridge
    Pages 139-157
  9. Michael L. Ziegler
    Pages 211-219
  10. Andrejs Liepins
    Pages 221-235
  11. Gerd G. Maul, Josef Weibel
    Pages 237-243
  12. Potu N. Rao, Prasad S. Sunkara, Abdullatif A. Al-Bader
    Pages 245-254
  13. Margaret J. Hightower, Joseph J. Lucas
    Pages 255-267

About this book

Introduction

Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito­ chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu­ lating following the characterization of cytoplasmic mutations. The produc­ tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.

Keywords

DNA chromosome gene expression genetics hybridization

Editors and affiliations

  • Jerry W. Shay
    • 1
  1. 1.The University of Texas Health Science Center at DallasDallasUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4684-4271-7
  • Copyright Information Springer-Verlag US 1982
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-4273-1
  • Online ISBN 978-1-4684-4271-7
  • About this book