Methods of Cell Separation

  • Nicholas Catsimpoolas

Part of the Biological Separations book series (BIOSEP)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Peter H. Bartels, George B. Olson
    Pages 1-99
  3. Jeane P. Hester, Robert M. Kellogg, Alfred Mulzet, Kenneth B. McCredie, Emil J. Freireich
    Pages 101-113
  4. Paul L. Kronick
    Pages 115-139
  5. Fabian J. Lionetti, Stephen M. Hunt, C. Robert Valeri
    Pages 141-156
  6. Chris D. Platsoucas, Nicholas Catsimpoolas
    Pages 157-200
  7. Back Matter
    Pages 201-203

About this book


Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu­ nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ­ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, spe­ cialization, interactions, cytotoxicity, and transformation. Clinical diagno­ sis of diseased states and use of isolated cells for therapeutic (e. g. , immu­ notherapy) or survival (e. g, transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However, the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-ended treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures presently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological problems.


X-ray cancer cancer research cell cells cognition cytotoxicity development erythrocyte image analysis instrumentation leukocyte microscope research toxicity

Editors and affiliations

  • Nicholas Catsimpoolas
    • 1
  1. 1.Biophysics Laboratory, Department of Nutrition and Food ScienceMassachusetts Institute of TechnologyCambridgeUSA

Bibliographic information