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Modern Applications of DNA Amplification Techniques

Problems and New Tools

  • Dirk Lassner
  • Barbara Pustowoit
  • Arndt Rolfs

Table of contents

  1. Front Matter
    Pages I-VIII
  2. PCR Quantification and Technical Aspects

    1. Front Matter
      Pages 1-1
    2. Tanya Vener, Malin Stark, Jan Albert, Mathias Uhlén, Joakim Lundeberg
      Pages 3-10
    3. Claus Stefan Vörtler, Klara Birikh
      Pages 47-54
    4. Dirk Lassner, Joerg Milde, Matthias Ladusch, Karl Droessler, Harald Remke
      Pages 55-63
    5. Volker Uhlmann, Eilhard Mix, Arndt Rolfs
      Pages 65-76
    6. Dani Bercovich, Zipi Regev, Tal Ratz, Yoram Plotsky
      Pages 83-89
  3. PCR Quantification of Infectious Agents

  4. Back Matter
    Pages 129-143

About this book

Introduction

In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.

Keywords

DNA ELISA Nucleotide PCR Polymerasekettenreaktion cloning genes polymer reaction regulation tissue transcription

Editors and affiliations

  • Dirk Lassner
    • 1
  • Barbara Pustowoit
    • 2
  • Arndt Rolfs
    • 3
  1. 1.Institute of Clinical Chemistry and PathobiochemistryUniversity of LeipzigLeipzigGermany
  2. 2.Institute of VirologyUniversity of LeipzigLeipzigGermany
  3. 3.Clinic and Policlinic of NeurologyUniversity of RostockRostockGermany

Bibliographic information