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Modern Applications of DNA Amplification Techniques

Problems and New Tools

  • Book
  • © 1997

Overview

Editors:
  1. Dirk Lassner

    1. Institute of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany

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  2. Barbara Pustowoit

    1. Institute of Virology, University of Leipzig, Leipzig, Germany

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  3. Arndt Rolfs

    1. Clinic and Policlinic of Neurology, University of Rostock, Rostock, Germany

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Table of contents (13 chapters)

  1. Front Matter

    Pages I-VIII
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  2. PCR Quantification and Technical Aspects

    1. Front Matter

      Pages 1-1
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    2. Multiple Competitors for Single-Tube Quantification of HIV-1 DNA

      • Tanya Vener, Malin Stark, Jan Albert, Mathias Uhlén, Joakim Lundeberg
      Pages 3-10

    3. Quantitation of P53 Tumor Suppressor Gene Copy Number in Tumor Dna Samples by Competitive PCR in an Elisa-Format

      • Meinhard Hahn, Volker Dörsam, Alfred Pingoud
      Pages 11-23

    4. Standardisation of Messenger RNA Quantification Using an RT-PCR Method Involving Co-Amplification with a Multi-Specific Internal Control

      • David Shire, Pascale Legoux, Adrian J. Minty
      Pages 25-35

    5. Quantitative Analysis of Human DNA Sequences by Solid-Phase Minisequencing

      • Ann-Christine Syvänen
      Pages 37-45

    6. Bioimage Analysers: Application for Ribozyme Kinetics

      • Claus Stefan Vörtler, Klara Birikh
      Pages 47-54

    7. First Approaches to Quantitate MDR1-Messenger RNA by In Cell PCR

      • Dirk Lassner, Joerg Milde, Matthias Ladusch, Karl Droessler, Harald Remke
      Pages 55-63

    8. Application of In Situ-PCR for the Detection of Intracellular mRNAs

      • Volker Uhlmann, Eilhard Mix, Arndt Rolfs
      Pages 65-76

    9. Psoralen Biotin: A Novel Reagent for Non-Enzymatic and Specific Labeling of Nucleic Acid Probes and Oligonucleotides

      • Robert L. Burghoff, Jens Beator, Michael A. Harvey
      Pages 77-82

    10. The Effect of Quantitative Ratio Between Primer Pairs on Pcr Products in Multi-Target Amplification

      • Dani Bercovich, Zipi Regev, Tal Ratz, Yoram Plotsky
      Pages 83-89

  4. Back Matter

    Pages 129-143
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Keywords

About this book

In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.

Editors and Affiliations

  • Institute of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany

    Dirk Lassner

  • Institute of Virology, University of Leipzig, Leipzig, Germany

    Barbara Pustowoit

  • Clinic and Policlinic of Neurology, University of Rostock, Rostock, Germany

    Arndt Rolfs

Bibliographic Information

  • Book Title: Modern Applications of DNA Amplification Techniques

  • Book Subtitle: Problems and New Tools

  • Editors: Dirk Lassner, Barbara Pustowoit, Arndt Rolfs

  • DOI: https://doi.org/10.1007/978-1-4615-5379-3

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1997

  • Hardcover ISBN: 978-0-306-45801-9Published: 31 December 1997

  • Softcover ISBN: 978-1-4613-7455-8Published: 23 October 2012

  • eBook ISBN: 978-1-4615-5379-3Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: VIII, 143

  • Number of Illustrations: 20 b/w illustrations

  • Topics: Animal Anatomy / Morphology / Histology, Biotechnology

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