Advertisement

Physicochemical Methods in the Study of Biomembranes

  • Herwig J. Hilderson
  • Gregory B. Ralston

Part of the Subcellular Biochemistry book series (SCBI, volume 23)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Gerrit van Meer, Ida L. van Genderen
    Pages 1-24
  3. Robert M. Johnson
    Pages 161-203
  4. Philip W. Kuchel, Kiaran Kirk, Glenn F. King
    Pages 247-327
  5. Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert
    Pages 329-362
  6. Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert
    Pages 363-403
  7. Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert
    Pages 405-450
  8. Back Matter
    Pages 493-509

About this book

Introduction

In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi­ tionally been applied to studies of model systems of lipids of well-defined com­ position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans­ port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.

Keywords

Biomembran Lipid membrane membrane transport protein proteins spectroscopy

Editors and affiliations

  • Herwig J. Hilderson
    • 1
  • Gregory B. Ralston
    • 2
  1. 1.University of AntwerpAntwerpBelgium
  2. 2.University of SydneySydneyAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4615-1863-1
  • Copyright Information Plenum Press, New York 1994
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-5757-5
  • Online ISBN 978-1-4615-1863-1
  • Series Print ISSN 0306-0225
  • Buy this book on publisher's site