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Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Book
  • © 2014
  • Latest edition

Overview

Editors:
  1. Shulin Li

    1. The University of Texas M.D. Anderson Cancer Center, Houston, USA

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  2. Jeffry Cutrera

    1. The University of Texas M.D. Anderson Cancer Center, Houston, USA

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  3. Richard Heller

    1. Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, USA

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  4. Justin Teissie

    1. IPBS CNR, Toulouse, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1121)

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About this book

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Basic Concepts and Enhancement of DNA Transfer via Electroporation

    1. Front Matter

      Pages 1-1
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    2. Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments

      • Lluis M. Mir
      Pages 3-23

    3. Electropermeabilization of the Cell Membrane

      • Justin Teissie
      Pages 25-46

    4. The Impact of Non-electrical Factors on Electrical Gene Transfer

      • Jiemiao Hu, Jeffry Cutrera, Shulin Li
      Pages 47-54

    5. Electroporation Formulation for Cell Therapy

      • Jiemiao Hu, Shulin Li
      Pages 55-60

    6. Short-Fragment DNA-Mediated In Vivo DNA Electroporation Delivery

      • Jinliang Peng, Yonggang Zhao, Yuhong Xu
      Pages 61-67

    7. Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection

      • Shuyan Huang, Yingbo Zu, Shengnian Wang
      Pages 69-77

  3. SiRNA Electrotransfer

    1. Front Matter

      Pages 79-79
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    2. siRNA Delivery via Electropulsation: A Review of the Basic Processes

      • Muriel Golzio, Justin Teissie
      Pages 81-98

    3. Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips

      • Zewen Wei, Zhihong Li
      Pages 99-110

    4. Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells

      • Isabel Siegert, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, Jonathan Jantsch
      Pages 111-119

    5. Direct Imaging of siRNA Electrotransfer at the Single-Cell Level

      • Justin Teissie, Muriel Golzio
      Pages 121-130

    6. Electroporation-Mediated siRNA Delivery into Tumors

      • Yoshifumi Takei
      Pages 131-138

    7. Substrate-Mediated, High-Efficiency siRNA Electroporation

      • Hiroyuki Fujimoto, Hiroo Iwata
      Pages 139-146

    8. Intradermal Electroporation of RNA

      • Maria L. Knudsen, Karl Ljungberg, Peter Liljeström, Daniel X. Johansson
      Pages 147-154

  4. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues

    1. Front Matter

      Pages 155-155
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    2. Delivery of DNA into the Central Nervous System via Electroporation

      • Jochen De Vry, Tim Vanmierlo, Pilar Martínez-Martínez, Mario Losen, Yasin Temel, Harry Steinbusch et al.
      Pages 157-163

    3. DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes

      • John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal et al.
      Pages 165-177

    4. Delivery of Plasmid DNA into Dental Tissues of Developing Rat Teeth by Electroporation

      • Shaomian Yao, Michael L. Beckley, Dawen Liu
      Pages 179-188
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Editors and Affiliations

  • The University of Texas M.D. Anderson Cancer Center, Houston, USA

    Shulin Li, Jeffry Cutrera

  • Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, USA

    Richard Heller

  • IPBS CNR, Toulouse, France

    Justin Teissie

Bibliographic Information

  • Book Title: Electroporation Protocols

  • Book Subtitle: Preclinical and Clinical Gene Medicine

  • Editors: Shulin Li, Jeffry Cutrera, Richard Heller, Justin Teissie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4614-9632-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4614-9631-1Published: 08 February 2014

  • Softcover ISBN: 978-1-4939-5492-6Published: 27 August 2016

  • eBook ISBN: 978-1-4614-9632-8Published: 08 February 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 349

  • Number of Illustrations: 20 b/w illustrations, 49 illustrations in colour

  • Topics: Human Genetics, Gene Expression

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