Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Shulin Li
  • Jeffry Cutrera
  • Richard Heller
  • Justin Teissie

Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Basic Concepts and Enhancement of DNA Transfer via Electroporation

  3. SiRNA Electrotransfer

    1. Front Matter
      Pages 79-79
    2. Isabel Siegert, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, Jonathan Jantsch
      Pages 111-119
    3. Justin Teissie, Muriel Golzio
      Pages 121-130
    4. Hiroyuki Fujimoto, Hiroo Iwata
      Pages 139-146
    5. Maria L. Knudsen, Karl Ljungberg, Peter Liljeström, Daniel X. Johansson
      Pages 147-154
  4. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues

    1. Front Matter
      Pages 155-155
    2. Jochen De Vry, Tim Vanmierlo, Pilar Martínez-Martínez, Mario Losen, Yasin Temel, Harry Steinbusch et al.
      Pages 157-163
    3. John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal et al.
      Pages 165-177
    4. Shaomian Yao, Michael L. Beckley, Dawen Liu
      Pages 179-188

About this book

Introduction

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Keywords

DNA transfer DNA vaccines SiRNA electrotransfer electroporation therapy gene delivery

Editors and affiliations

  • Shulin Li
    • 1
  • Jeffry Cutrera
    • 2
  • Richard Heller
    • 3
  • Justin Teissie
    • 4
  1. 1.The University of Texas M.D. Anderson Cancer CenterHoustonUSA
  2. 2.The University of Texas M.D. Anderson Cancer CenterHoustonUSA
  3. 3.Old Dominion University Frank Reidy Ctr for BioelectricsNorfolkUSA
  4. 4.IPBS CNRToulouseFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4614-9632-8
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4614-9631-1
  • Online ISBN 978-1-4614-9632-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book