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The Low Molecular Weight Proteome

Methods and Protocols

  • Helena Bäckvall
  • Janne Lehtiö

Part of the Methods in Molecular Biology book series (MIMB, volume 1023)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Low Molecular Weight Analysis Methods

    1. Front Matter
      Pages 1-1
    2. Johan Lengqvist, AnnSofi Sandberg
      Pages 21-51
    3. Michael A. Kuzyk, Carol E. Parker, Dominik Domanski, Christoph H. Borchers
      Pages 53-82
    4. Matthias Rainer, Constantin Sajdik, Günther K. Bonn
      Pages 83-95
    5. Brinda Shah, Jennifer D. Reid, Michael A. Kuzyk, Carol E. Parker, Christoph H. Borchers
      Pages 97-120
    6. Anna Ljungdahl, Jörg Hanrieder, Jonas Bergquist, Malin Andersson
      Pages 121-136
    7. Kimi Drobin, Peter Nilsson, Jochen M. Schwenk
      Pages 137-145
  3. Bioinformatics of Proteomics Data

  4. Methods Applied in Clinical Research

  5. Back Matter
    Pages 219-223

About this book

Introduction

Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress  adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and proteolytic fragments of larger proteins. Due to the unique features of these proteins, the technical challenges are different from those in ”common” proteomics.  In The Low Molecular Weight Proteome: Methods and Protocols expert researchers from the field provide protocols for analysis of low molecular weight proteins and peptides, protocols for such methods applied in clinical research and an up-to-date review of quantitative protein profiling by labeling.  These include methods suitable for both peptide and protein analysis with focus on methods and application that can be used for small protein analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, The Low Molecular Weight Proteome: Methods and Protocolsis a useful resource for experienced proteomics practitioners as well as an aid to newcomers who wish to become acquainted with the theory and practice of a wide array of methods in analyzing small proteins or peptides.

Keywords

MHC ligand identification MS based proteomics PQPQ-algorithm SELDI-MS in vitro phage display low-molecular-weight proteome peptides peptidomics proteomics small protein

Editors and affiliations

  • Helena Bäckvall
    • 1
  • Janne Lehtiö
    • 2
  1. 1., Department of Oncology-PathologyKarolinska InstitutetStockholmSweden
  2. 2., Department of Oncology-PathologyKarolinska InstitutetStockholmSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4614-7209-4
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4614-7167-7
  • Online ISBN 978-1-4614-7209-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site