Intrinsically Disordered Protein Analysis

Volume 2, Methods and Experimental Tools

  • Vladimir N. Uversky
  • A. Keith Dunker

Part of the Methods in Molecular Biology book series (MIMB, volume 896)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Single Molecule Techniques

    1. Front Matter
      Pages 1-1
    2. Benjamin Schuler, Sonja Müller-Späth, Andrea Soranno, Daniel Nettels
      Pages 21-45
    3. Marco Brucale, Isabella Tessari, Luigi Bubacco, Bruno Samorì
      Pages 47-56
    4. Toshio Ando, Noriyuki Kodera
      Pages 57-69
    5. Javier Oroz, Rubén Hervás, Alejandro Valbuena, Mariano Carrión-Vázquez
      Pages 71-87
  3. Methods to Assess Protein Size and Shape

  4. Methods to Analyze Conformational Behavior

    1. Front Matter
      Pages 195-195
    2. Paolo Neyroz, Stefano Ciurli, Vladimir N. Uversky
      Pages 197-213
    3. Matthew D. Smith, Masoud Jelokhani-Niaraki
      Pages 223-231
    4. Magnus Kjaergaard, Flemming M. Poulsen, Birthe B. Kragelund
      Pages 233-247

About this book


Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions. In Intrinsically Disordered Protein Analysis: Methods and Experimental Tools, expert researchers explore the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. Volume 2 includes sections on single molecule techniques, methods to assess protein size and shape, analyzing conformational behavior, mass-spectrometry, expression and purification of IDP’s. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Intrinsically Disordered Protein Analysis: Methods and Experimental Tools helps scientists further their investigations of these fascinating and dynamic molecules.


functional analysis globular proteins intrinsically disordered proteins physico-chemical protein function protein self-organization structural analysis unfolded proteins unfoldome

Editors and affiliations

  • Vladimir N. Uversky
    • 1
  • A. Keith Dunker
    • 2
  1. 1.DEPT OF MOLECULAR MED, College of MedicineUniversity of South FloridaTAMPAUSA
  2. 2.Dept. Biochemistry & Molecular Biology, Center for Computational Biology &Indiana UniversityIndianapolisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4614-3703-1
  • Online ISBN 978-1-4614-3704-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book