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Mass Spectrometry in Drug Metabolism

  • Alberto Frigerio
  • Emilio L. Ghisalberti

Table of contents

  1. Front Matter
    Pages i-xii
  2. Identification of Drugs and Drug Metabolites

    1. A. Donetti, L. G. Dring, P. C. Hirom, R. T. Williams
      Pages 1-11
    2. D. Pitrè, A. Frigerio, R. Maffei-Facino
      Pages 13-30
    3. H. R. Sullivan, R. E. McMahon, D. G. Hoffman, S. Ridolfo
      Pages 31-43
    4. G. Pifferi, P. Ventura, C. Farina, A. Frigerio
      Pages 45-59
    5. R. Caponi, R. Fumagalli, S. Innocenti, P. Martelli, U. Valcavi
      Pages 61-72
    6. I. Matsumoto, T. Kuhara, M. Yoshino, M. Tetsuo
      Pages 73-80
    7. E. Grassi, G. L. Passetti, A. Trebbi, A. Frigerio
      Pages 95-110
    8. E. L. Ghisalberti, P. R. Jefferies, R. Lanteri
      Pages 111-130
  3. Quantitation of Drugs and Drug Metabolites

  4. Intermediates in the Metabolism of Drugs

  5. Stable Isotope Labelling

  6. Developments in Methodology

  7. Drug Abuse

  8. Back Matter
    Pages 503-532

About this book

Introduction

When a dose of drug is administered, three main phases of drug action may be distingushed. In the "pharmaceutical" phase the dosage form disintegrates, the active substance dissolves and becomes available for absorption. The second phase ("pharmaco­ kinetic" phase) includes absorption, distribution, metabolism, and excretion. That fraction of the dose which finally reaches the circulation after absorption will be available for biological action in the third, or "pharmacodynamic" phase when the drug reaches the target tissues and a drug-receptor interaction takes place. The objectives in studies of drug metabolism are: (a) to identify the pathways by which drugs are transformed in the body; (b) to ascertain quantitatively the importance of each pathway and intermediate; (c) to identify and quantify endogenous constituents influenced by the drug or its metabolites which may interfere with common metabolic processes. Since metabolites usually differ from their precursors by only a single chemical group, the resulting metabolic pathways generally consist of a series of closely related compounds. Mass spectrometry is uniquely suited for the analysis of drugs and metabolites for several reasons: only a minimal amount of sample preparation is needed, closely related compounds can be analyzed in a single step, structures can often be deduced directly from the mass spectra without the need for pure reference spectra, and constituents can be quantified with relative ease even when present in fractional nanogram quantity.

Keywords

Alanin Mass Spectrometry absorption cannabinoid depression drug drug abuse drug research environment metabolism protein research tissue

Editors and affiliations

  • Alberto Frigerio
    • 1
  • Emilio L. Ghisalberti
    • 2
  1. 1.“Mario Negri” Institute for Pharmacological ResearchMilanItaly
  2. 2.Department of Organic ChemistryUniversity of Western AustraliaNedlandsAustralia

Bibliographic information