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Proteins

Structure and Function

  • Book
  • © 1987

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Editors:
  1. James J. L’Italien

    1. Molecular Genetics, Inc, Minnetonka, USA

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Table of contents (82 chapters)

  1. Methods of Polypeptide Purification and Characterization

    2. Amino Acid Analysis of Polypeptides

      1. A Practical Guide to the General Application of PTC-Amino Acid Analysis
        • Robert L. Heinrikson, Rene Mora, John M. Maraganore
        Pages 187-195
      2. High Performance Liquid Chromatography of Phenylthiohydantoin and Phenylthiocarbamyl Amino Acids
        • Barry N. Jones, Angelo P. Consalvo, Lisa LeSueur, Susan Lovato, Stanley D. Young, James A. Koehn et al.
        Pages 197-205
      3. Amino Acid Analysis Using Pre-Column Derivatization with Phenylisothiocyanate: Matrix Effects and Tryptophan Analysis
        • Steven A. Cohen, Thomas L. Tarvin, Brian A. Bidlingmeyer
        Pages 207-213
      4. An Improved Reverse Phase HPLC Separation and Postcolumn Detection of Amino Acids
        • Stephen Gruber, Noel M. Meltzer, Stanley Stein, Guillermo I. Tous
        Pages 215-223
      5. HPLC Assay of Phosphoamino Acids by Fluorescence Detection
        • Michael J. Watson, Joan R. Kanter, Laurence L. Brunton
        Pages 225-231
      6. Amino Acid Analysis by Reverse-Phase High Performance Liquid Chromatography: Separation of Phenylthiocarbamyl Amino Acids by Spherisorb Octadecylsilane Columns
        • Chao-yuh Yang, Felix I. Sepulveda, Tseming Yang, Wei-yong Huang
        Pages 233-238

    3. Mass Spectrometry of Polypeptides

      1. Fast Atom Bombardment Mass Spectrometry: Application to Peptide Structural Analysis
        • Blair A. Fraser
        Pages 241-249
      2. Protein Sequence Determination and FAB Mass Spectrometry
        • Terry D. Lee, Kassu Legesse, Vickie Spayth
        Pages 251-268
      3. The Use of HPLC and Mass Spectrometric Techniques to Quantify Endogenous Opioid Peptides
        • Dominic M. Desider, Genevieve H. Fridland, F. S. Tanzer, Chhabil Dass, Peter Tinsley, John Killmar
        Pages 269-282
      4. Structural Analysis of Proteins of the Nervous System
        • Daniel R. Marshak
        Pages 283-298
      5. Pancreatic Preprosomatostatin Processing: Isolation and Structure of Intermediates and Final Products of Processing
        • P. C. Andrews, J. E. Dixon
        Pages 299-304

    4. Microsequence Analysis of Polypeptides by Edman Degraduation

      1. Progress Toward Polybrene Purification and Utility in Micro-Peptide/Protein Sequencing
        • Pau M. Yuan, Sylvia Yuen, John Bergot, Michael W. Hunkapiller, Kenneth J. Wilson
        Pages 307-313
      2. Approaches for Microsequencing: Extraction from SDS Gel, Composition, Terminal Analysis
        • Akira Tsugita, Tatsukai Ataka
        Pages 315-322
      3. Manual Gas Phase Microsequencing on Filterchips for Simultaneous Edman Degradation of Multiple Peptide Samples
        • E. L. Mehl, M. Haniu, J. E. Shively
        Pages 323-332
      4. Strategies for Sequencing Peptide Mixtures by Selective Blocking
        • Michael N. Margolies, Andrew W. Brauer, Rou-Fun Kwong, Gary R. Matsueda
        Pages 333-341
      5. Amidation of Protein Carboxyl Groups
        • George E. Tarr
        Pages 343-353
      6. A Method for Direct Amino Acid Sequence Analysis of the NH2-Terminal Regions of Fibrinogen
        • Chung Y. Liu, Francis J. Morgan
        Pages 355-360

    5. Analysis of PTH Amino Acids

      1. PTH Amino Acid Analysis
        • Michael W. Hunkapiller
        Pages 363-381
      2. An On-Line Isocratic HPLC System for the Analysis of PTH-Amino Acids on A Gas-Phase Sequencer
        • J. E. Shively, D. Hawke, R. M. Kutny, B. Krieger, J. L. Glajch
        Pages 383-394
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Keywords

About this book

This volume surveys the current status of many of the important methods and approaches which are central to the study of protein structure and function. Many of the articles in this volume are written to emphasize the general utility of the method or approach which is at its core, and to provide sufficient literature references to enable the reader to adapt the method or approach to other applications. It is hoped that this volume will provide a source from which newcomers as well as experienced scient­ ists may becom& more familiar with recent developments and future trends in some of the important areas of protein research. The articles which comprise this book are selected proceedings from the Symposium of American Protein Chemists, which was held in San Diego, California, September 30 to October 3, 1985. The goal of the organizers of this first symposium was to provide a forum for discussion and inter­ action among scientists whose interests span the broad spectrum of protein structure and function research. The concept and timing of the symposium well received as evidenced by the approximately 500 delegates to the was symposium. The inaugural meeting was marked by a strong scientific pro­ gram with over 140 papers presented in either a lecture or poster format.

Editors and Affiliations

  • Molecular Genetics, Inc, Minnetonka, USA

    James J. L’Italien

Bibliographic Information

  • Book Title: Proteins

  • Book Subtitle: Structure and Function

  • Editors: James J. L’Italien

  • DOI: https://doi.org/10.1007/978-1-4613-1787-6

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Plenum Press, New York 1987

  • Softcover ISBN: 978-1-4612-9001-8Published: 10 November 2011

  • eBook ISBN: 978-1-4613-1787-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 834

  • Topics: Biochemistry, general

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