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Proteins

Structure and Function

  • James J. L’Italien

Table of contents

  1. Front Matter
    Pages iii-xv
  2. Methods of Polypeptide Purification and Characterization

    1. Front Matter
      Pages 1-1
    2. Chromatographic Methods of Polypeptide Purification

      1. Kenneth J. Wilson, David R. Dupont, Pau M. Yuan, Michael W. Hunkapiller, Timothy D. Schlabach
        Pages 21-28
      2. Petro E. Petrides, Peter Bohlen, Frederick S. Esch
        Pages 29-36
      3. Edouard C. Nice, John A. Smith, Robert L. Moritz, Michael J. O’Hare, Philip S. Rudland, John R. Morrison et al.
        Pages 37-44
      4. K. R. Williams, K. L. Stone, M. K. Fritz, B. M. Merrill, W. H. Konigsberg, M. Pandolfo et al.
        Pages 45-52
      5. J. L. Wittliff, N. A. Shahabi, S. M. Hyder, L. A. van der Walt, L. Myatt, D. M. Boyle et al.
        Pages 61-74
      6. Robert C. Sullivan, John A. Smith, Ricky Nelson, Yuen W. Shing, Michael Klagsbrun
        Pages 75-81
    3. Electrophortic Methods of Polypeptide Purification

    4. Chemical Modification of Polypeptides

    5. Amino Acid Analysis of Polypeptides

      1. Robert L. Heinrikson, Rene Mora, John M. Maraganore
        Pages 187-195
      2. Barry N. Jones, Angelo P. Consalvo, Lisa LeSueur, Susan Lovato, Stanley D. Young, James A. Koehn et al.
        Pages 197-205
      3. Stephen Gruber, Noel M. Meltzer, Stanley Stein, Guillermo I. Tous
        Pages 215-223
      4. Michael J. Watson, Joan R. Kanter, Laurence L. Brunton
        Pages 225-231
    6. Mass Spectrometry of Polypeptides

      1. Terry D. Lee, Kassu Legesse, Vickie Spayth
        Pages 251-268
      2. Dominic M. Desider, Genevieve H. Fridland, F. S. Tanzer, Chhabil Dass, Peter Tinsley, John Killmar
        Pages 269-282
    7. Microsequence Analysis of Polypeptides by Edman Degraduation

      1. Pau M. Yuan, Sylvia Yuen, John Bergot, Michael W. Hunkapiller, Kenneth J. Wilson
        Pages 307-313
      2. Michael N. Margolies, Andrew W. Brauer, Rou-Fun Kwong, Gary R. Matsueda
        Pages 333-341
      3. George E. Tarr
        Pages 343-353
    8. Analysis of PTH Amino Acids

    9. Computer Analysis of Protein Sequence Data

    10. Miscellaneous Methods of Polypeptide Characterization

      1. Richard A. Houghten, Sarah R. Hoffmann, Mairead K. Bray, Nicole Frizzell, J. M. Ostresh, Suzanne M. Pratt et al.
        Pages 463-470
      2. Gregory A. Grant, Vishva M. Dixit, Nancy J. Galvin, Karen M. O’Rourke, Samuel A. Santoro, William A. Frazier
        Pages 471-477
      3. Betsy J. Bricker, Robert R. Wagner, Jay W. Fox
        Pages 479-486

About this book

Introduction

This volume surveys the current status of many of the important methods and approaches which are central to the study of protein structure and function. Many of the articles in this volume are written to emphasize the general utility of the method or approach which is at its core, and to provide sufficient literature references to enable the reader to adapt the method or approach to other applications. It is hoped that this volume will provide a source from which newcomers as well as experienced scient­ ists may becom& more familiar with recent developments and future trends in some of the important areas of protein research. The articles which comprise this book are selected proceedings from the Symposium of American Protein Chemists, which was held in San Diego, California, September 30 to October 3, 1985. The goal of the organizers of this first symposium was to provide a forum for discussion and inter­ action among scientists whose interests span the broad spectrum of protein structure and function research. The concept and timing of the symposium well received as evidenced by the approximately 500 delegates to the was symposium. The inaugural meeting was marked by a strong scientific pro­ gram with over 140 papers presented in either a lecture or poster format.

Keywords

Amino acid Nucleotide Purine enzymes primary structure protein sequence protein structure

Editors and affiliations

  • James J. L’Italien
    • 1
  1. 1.Molecular Genetics, IncMinnetonkaUSA

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