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  • © 2003

Biological Electron Microscopy

Theory, Techniques, and Troubleshooting

  • Covers a wide range of topics including conventional light microscopy, transmission electron microscopy, and scanning electron microscopy

  • Includes a survey of each subject, and a techniques section, where appropriate, with tried-and-true methods that will produce publishable results

  • Serves as a broadly applicable starting point for a variety of approaches to cytological research

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Table of contents (32 chapters)

  1. Front Matter

    Pages i-xviii
  2. Specimen Preparation for Electron Microscopy

    • Michael J. Dykstra, Laura E. Reuss
    Pages 1-73
  3. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 74-123
  4. Cryotechniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 125-149
  5. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 150-152
  6. Ultramicrotomy

    • Michael J. Dykstra, Laura E. Reuss
    Pages 153-158
  7. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 159-174
  8. Staining Methods for Semithins and Ultrathins

    • Michael J. Dykstra, Laura E. Reuss
    Pages 175-189
  9. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 190-196
  10. Cytochemistry

    • Michael J. Dykstra, Laura E. Reuss
    Pages 197-208
  11. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 209-217
  12. Immunocytochemistry

    • Michael J. Dykstra, Laura E. Reuss
    Pages 219-231
  13. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 232-239
  14. Support Films

    • Michael J. Dykstra, Laura E. Reuss
    Pages 241-244
  15. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 245-258
  16. Replicas, Shadowing, and Negative Staining

    • Michael J. Dykstra, Laura E. Reuss
    Pages 259-270
  17. Techniques

    • Michael J. Dykstra, Laura E. Reuss
    Pages 271-285
  18. Transmission Electron Microscopy

    • Michael J. Dykstra, Laura E. Reuss
    Pages 287-322
  19. Vacuum Systems

    • Michael J. Dykstra, Laura E. Reuss
    Pages 323-337
  20. High-Voltage Transmission Electron Microscopes (HVEM)

    • Michael J. Dykstra, Laura E. Reuss
    Pages 339-345

About this book

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their partiĀ­ cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instruĀ­ ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Keywords

  • Fixation
  • electron microscopy
  • microscopy
  • scanning electron microscope
  • transmission electron microscopy
  • Biological Microscopy

Reviews

"In this second edition of his 1992 hardcover text and 1993 spiral-bound lab manual on Biological Electron Microscopy, Michael Dykstra has expended considerable effort to merge the two earlier volumes into a more readable and usable single volume and also to update them and add considerable new material. Happily, the result is a first rate, comprehensive book that will be useful for both teaching beginning students and as a reference book for experienced researchers.
In all chapters, new materials have been added in the text and referenced at the chapter ends, and where appropriate, useful web sites have been indicated where additional information may be obtained. All of the excellent illustrative photographs from the first edition have been retained. As before, the chapters on microscope construction and operation are very well done and will be invaluable in teaching. Cautionary statements are made throughout about handling and disposal of the hazardous materials used in EM labs. Relevant journals, societies, and equipment suppliers are listed in appendices.
In general, this single volume is a welcome addition to the literature available on biological microscopy. Its publication offers support for the idea that microscopy in all of its guises is still a dynamic and valuable tool for all biologists."
(Henry C. Aldrich, Professor Emeritus of Microbiology and Cell Science, University of Florida, Gainesville)

Authors and Affiliations

  • North Carolina State University, USA

    Michael J. Dykstra, Laura E. Reuss

Bibliographic Information

  • Book Title: Biological Electron Microscopy

  • Book Subtitle: Theory, Techniques, and Troubleshooting

  • Authors: Michael J. Dykstra, Laura E. Reuss

  • DOI: https://doi.org/10.1007/978-1-4419-9244-4

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Michael J. Dykstra 2003

  • Hardcover ISBN: 978-0-306-47749-2Published: 31 December 2004

  • Softcover ISBN: 978-1-4613-4856-6Published: 20 November 2012

  • eBook ISBN: 978-1-4419-9244-4Published: 28 June 2011

  • Edition Number: 2

  • Number of Pages: XVIII, 534

  • Topics: Biological Microscopy, Life Sciences, general, Veterinary Medicine/Veterinary Science, Microbiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access