A Beginner’s Guide to Microarrays

  • Eric M. Blalock

Table of contents

About this book

Introduction

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:

-Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols. 

Keywords

DNA Nucleotide RNA clustering data analysis database gene expression genes performance

Editors and affiliations

  • Eric M. Blalock
    • 1
  1. 1.University of Kentucky Medical CenterUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4419-8760-0
  • Copyright Information Kluwer Academic Publishers 2003
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-4684-5
  • Online ISBN 978-1-4419-8760-0
  • About this book