Evolving Methods for Macromolecular Crystallography

1. Front Matter

   Pages i-viii

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2. Succeeding with seeding: some practical advice

   • Terese Bergfors

   Pages 1-10

3. Expression, purification, and crystallisationof membrane proteins

   • Bernadette Byrne

   Pages 11-23

4. Macromolecular cryo-crystallography

   • Elspeth Garman

   Pages 25-40

5. Processing diffraction data with mosflm

   • Andrew G. W. Leslie, Harold R. Powell

   Pages 41-51

6. Sad phasing: basic concepts and high-throughput

   • George M. Sheldrick

   Pages 53-65

7. Likelihood-based experimental phasing in phaser

   • Airlie J. McCoy, Laurent C. Storoni, Randy J. Read

   Pages 67-77

8. Stochastic molecular replacement

   • Nicholas M. Glykos

   Pages 79-90

9. Likelihood-based molecular replacement in phaser

   • Randy J. Read, Airlie J. McCoy, Laurent C. Storoni

   Pages 91-100

10. Automated structure determination with phenix

    • Tod D. Romo, James C. Sacchettini, Thomas C. Terwilliger, Paul D. Adams, Pavel V. Afonine, Ralf W. Grosse-Kunstleve et al.

    Pages 101-109

11. Density Modification In Main

    • Dušan Turk

    Pages 111-122

12. Ab initio phasing starting from low resolution

    • Vladimir Lunin, Natalia Lunina, Alexandre Urzhumtsev

    Pages 123-133

13. Structural genomics of mycobacterium tuberculosis: a search for function and new drug targets

    • Ted Baker

    Pages 135-144

14. Three-dimensional domain swapping and its relevance to conformational diseases

    • Mariusz Jaskolski

    Pages 145-163

15. Structural bioinformatics: from protein structure to function

    • Aled M. Edwards, James D. Watson, Adel Golovin, Roman A. Laskowski, Kim Henrick, Janet M. Thornton et al.

    Pages 165-179

16. Single-particle imaging

    • David Sayre

    Pages 181-191

This volume comprises papers presented at the 2005 edition of the “Crystallography of Molecular Biology” courses that have been held since 1976 at the Ettore Majorana Centre for Scientific Culture in Erice, Italy. This series of courses is renowned for bringing leaders in the field of macromo- cular crystallography together with highly motivated students, in a beautiful and intimate location that encourages people to interact. The warm and informal atmosphere at these Erice conferences, especially these on cryst- lography, has helped to foster long-term scientific interactions and new int- national friendships that have often lasted for the lifetime of the scientists. The course was financed by NATO as an Advanced Study Institute and by the European Commission as a EuroSummerSchool. The papers span the breadth of material presented in the course, which emphasized the practical aspects of modern macromolecular crystallography and its applications. One must start with crystals: Bergfors showed how to improve initial crystals through seeding, while Byrne discussed the difficult problem of crystallizing membrane proteins. The collection of optimal diffraction data requires both careful preparation of cryo-cooled crystals (Garman) and proper processing of the diffraction images (Leslie). To obtain images of electron density, one needs estimates of the phases of the diffracted spots. Sheldrick presented the background to the sing- wavelength anomalous diffraction (SAD) method, which has been gaining popularity, and McCoy discussed the basis of modern maximum likelihood methods for treating information in experimental phasing.

• 3D
• Crystallography
• X-ray
• bioinformatics
• biology
• genome
• imaging
• proteins

From the reviews:

"The book is a summary of the proceedings on ‘Evolving Methods for Macromolecular Crystallography’ held in 2005. … it is a welcome addition to the crystallographer’s bookshelf with contributions by a number of experts in their field." (Miroslav Papiz, Crystallography Reviews, Vol. 15 (2), 2009)

• University of Cambridge, UK

  Randy J. Read

• Weizmann Institute of Science, Israel

  Joel L. Sussman

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