Reviews and Protocols in DT40 Research

Subcellular Biochemistry

  • Jean-Marie Buerstedde
  • Shunichi Takeda

Table of contents

  1. Front Matter
    Pages i-xii
  2. Jean-Marie Buerstedde, Hiroshi Arakawa
    Pages 11-24
  3. Randolph B. Caldwell, Andrzej M. Kierzek
    Pages 25-37
  4. William R. A. Brown, Margaret C. M. Smith, Felix Dafhnis-Calas, Sunir Malla, Zhengyao Xu
    Pages 39-48
  5. Masayuki Seki, Shusuke Tada, Takemi Enomoto
    Pages 49-73
  6. Sandrine Ruchaud, Kumiko Samejima, Damien Hudson, Scott H. Kaufmann, William C. Earnshaw
    Pages 75-90
  7. Masahiro Okada, Tetsuya Hori, Tatsuo Fukagawa
    Pages 91-106
  8. Michael D. Rainey, George Zachos, David A. F. Gillespie
    Pages 107-117
  9. Frank R. Wettey, Antony P. Jackson
    Pages 119-143
  10. Hisaaki Shinohara, Tomohiro Kurosaki
    Pages 145-187
  11. Jukka Alinikula, Olli Lassila, Kalle-Pekka Nera
    Pages 189-205
  12. Hidehiko Kikuchi, Hirak Kumar Barman, Masami Nakayama, Yasunari Takami, Tatsuo Nakayama
    Pages 225-243
  13. Paul E. Neima, Joan Burnside, Katrina Elsaesser, Harry Hwang, Bruce E. Clurman, Robert Kimmel et al.
    Pages 245-256
  14. Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg
    Pages 257-270
  15. Julian E. Sale, Anna-Laura Ross, Laura J. Simpson
    Pages 271-294
  16. Minoru Takata, Kazuhiko Yamamoto, Nobuko Matsushita, Hiroyoki Kitao, Sciki Hirano, Masamichi Ishaiai
    Pages 295-311
  17. Helfrid Hochegger, Shunichu Takeda
    Pages 313-325
  18. Ken-ichi Yamamoto, Masahiko Kobayashi, Hiroko Shimizu
    Pages 327-339

About this book


Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.


Calcium Chaperone DNA Endoplasmatisches Reticulum Microarray PCR RNA Termination biochemistry cell lines enzymes gene expression hybridization regulation transcription

Editors and affiliations

  • Jean-Marie Buerstedde
    • 1
  • Shunichi Takeda
    • 2
  1. 1.GSF National Research CentreNeuherbergGermany
  2. 2.Kyoto UniversityJapan

Bibliographic information

  • DOI
  • Copyright Information Springer 2006
  • Publisher Name Springer, Dordrecht
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-1-4020-4895-1
  • Online ISBN 978-1-4020-4896-8
  • About this book