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Neuronal Morphogenesis

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Kazuhito Toyooka

    1. Neurobiology and Anatomy, Drexel University, Philadelphia, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2831)

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About this book

This volume details molecular mechanisms that are involved in neuronal morphogenesis, and methods for analyzing these mechanisms. Chapters detail analyzing neuronal morphology in vitro and in vivo using a variety of model systems, including rodents and human induced pluripotent stem cells (hiPSCs). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Neuronal Morphogenesis: Methods and Protocols aims to be  suitable for a wide range of readers interested in the mechanisms of neuronal morphology and related neurological disorders.

Keywords

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Culturing Primary Cortical Neurons for Live-Imaging

    • Kyle R. Northington, Emily Anne Bates
    Pages 1-9

  3. Electron Microscopy of Neurons on Biomimetic Substrates

    • Claudia Latte Bovio, Valentina Mollo, Anna Mariano, Francesca Santoro
    Pages 11-20

  4. Analysis of Neurite and Spine Formation in Neurons In Vitro

    • Jenny R. Diaz, Martin J. Sadowski
    Pages 21-37

  5. Quantifying Dendritic Arbors In Vitro and In Vivo in Rodent Models

    • Rebecca J. Wilson, Jessie R. Badley, Pamela J. Lein
    Pages 39-57

  6. Visualizing and Measuring Dendrite Arborization in Drosophila Somatosensory Neurons

    • Yineng Xu, Inle Bush, Chun Han
    Pages 59-71

  7. Analyses of Neurite and Spine Formation by In Utero Electroporation in Mice

    • Hidenori Tabata, Nanako Hamada, Koh-ichi Nagata
    Pages 73-80

  8. Analysis of Neuronal Morphology by Two-Photon Microscopy

    • Chia-Wei Huang, Yen-Lin Su, Jin-Wu Tsai
    Pages 81-95

  9. In Ovo Electroporation of Embryonic Chicken Spinal Cord in Combination with Ex Vivo Slice Culture for Live Imaging of Vertebrate Neuronal Differentiation

    • Victoria E. Higgs, Gabriela Toro-Tapia, Holly B. Burbidge, Raman M. Das
    Pages 97-111

  10. In Vitro and In Vivo Analysis of Neuronal Polarity

    • Mini Jose
    Pages 113-132

  11. Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation

    • Kanika Sharma, Prashant Kaushal, Vikas Kumar
    Pages 133-143

  12. 3D Reconstruction of the Mitochondrial Network within the Neuronal Soma from SBF-SEM Volume Data

    • Jane Tweedy, Ross Laws, George Merces, Tracey Davey, Amy K. Reeve, Amy E. Vincent
    Pages 145-177

  13. DNeuroMAT: A Deep-Learning-Based Neuron Morphology Analysis Toolbox

    • Min Liu, Zhuangdian Lin, Weixun Chen, Erik Meijering, Yaonan Wang
    Pages 179-197

  14. Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation

    • Xiaonan Liu, Kazuhito Toyo-oka
    Pages 199-208

  15. Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy

    • Takeo Saneyoshi, Yasunori Hayashi
    Pages 209-217

  16. Visualization and Quantification of Organelle Axonal Transport in Cultured Neurons

    • Jayne Aiken, Erika L. F. Holzbaur
    Pages 219-234

  17. Analysis of Microtubule Polymerization During Axon Outgrowth Using Fluorescently Labeled Microtubule Plus-End Tracking Proteins

    • Isabel Pérez-Ferrer, Carlos Sánchez-Huertas
    Pages 235-249

  18. Interrogating the Molecular Clutch in Neuronal Growth Cones: Measuring Traction Forces, F-actin Retrograde Flow, and Point Contact Demographics

    • Ketakee Ghate, Sampada P. Mutalik, Aurnab Ghose
    Pages 251-264

  19. Measuring Retrograde Actin Flow in Neuronal Growth Cones

    • Laura Pulido Cifuentes, Daniel M. Suter
    Pages 265-282

  20. Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers

    • Osvaldo A. Miranda, Giselle Cheung, Simon Hippenmeyer
    Pages 283-299
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Editors and Affiliations

  • Neurobiology and Anatomy, Drexel University, Philadelphia, USA

    Kazuhito Toyooka

Bibliographic Information

  • Book Title: Neuronal Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Kazuhito Toyooka

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3969-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3968-9Published: 13 August 2024

  • Softcover ISBN: 978-1-0716-3971-9Due: 27 August 2025

  • eBook ISBN: 978-1-0716-3969-6Published: 12 August 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 383

  • Number of Illustrations: 3 b/w illustrations, 54 illustrations in colour

  • Topics: Neurosciences

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