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Connexin Hemichannels

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Fabio Mammano

    1. Department of Physics, University of Padova, Padova, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Mauricio Retamal

    1. Facultad de Medicina, Universidad del Desarrollo, Santiago, Chile

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2801)

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About this book

This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using confocal microscopy; evaluation of connexin HC activity in vivo; generation of connexin-expressing stable cell pools; and automated assessment of cutaneous pathology in a mouse model of HC dysfunction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Connexin Hemichannels: Methods and Protocols is a valuable resource that discusses the importance of HCs as drug targets, and how studying them is essential to identifying newcompounds capable of modulating HC function selectively and specifically.




Keywords

Table of contents (14 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels

    • Mariana C. Fiori, Guillermo A. Altenberg
    Pages 1-16

  3. Expression and Function of Connexins in Extracellular Vesicles

    • Tânia Martins-Marques, Henrique Girão
    Pages 17-28

  4. Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Characterization by Cryo-Electron Microscopy

    • Pia Lavriha, Chao Qi, Volodymyr M. Korkhov
    Pages 29-43

  5. Molecular Dynamics Simulation of Permeation Through Connexin Channels

    • Francesco Zonta, Fabio Mammano, Sergio Pantano
    Pages 45-56

  6. Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy

    • Sergiu A. Lucaciu, Stephanie E. Leighton, Dale W. Laird
    Pages 57-74

  7. Assessment of Connexin43 Hemichannel Functionality Based on Cytosolic Uptake of Yo-Pro1

    • Raf Van Campenhout, Julen Sanz-Serrano, Axelle Cooreman, Yannick De Vlaeminck, Karine Breckpot, Prashant Kadam et al.
    Pages 75-85

  8. A Dye Uptake Assay to Measure Large-Pore Channel Activity in Trypanosoma cruzi

    • José Luis Vega, Aníbal García, Jorge González, Juan C. Sáez
    Pages 87-95

  9. Measurement of Ca2+ Uptake Through Connexin Hemichannels

    • Chiara Nardin, Fabio Mammano
    Pages 97-109

  10. Evaluation of Connexin Hemichannel Activity In Vivo

    • Francisca M. Acosta, Manuel A. Riquelme, Sumin Gu, Jean X. Jiang
    Pages 111-124

  11. Protocol for the Study of Connexin and DNA Interactions

    • Ainoa Fernandez-Olivares, Mauricio A. Retamal
    Pages 125-134

  12. Enhanced Methodologies for Investigating the Electrophysiological Characteristics of Endogenous Pannexin 1 Intercellular Cell–Cell Channels

    • Nicolás Palacios-Prado, Teresa Vergara, Juan C. Sáez
    Pages 135-145

  13. Generation of Connexin-Expressing Stable Cell Pools

    • Abraham Tettey-Matey, Chiara Di Pietro, Viola Donati, Fabio Mammano, Daniela Marazziti
    Pages 147-176

  14. A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfunction

    • Chiara Peres, Fabio Mammano
    Pages 177-187

  15. Measuring Connexin Hemichannel Opening in Response to an InsP3-Mediated Cytosolic Ca2+ Increase

    • Erva Bayraktar, Mario Bortolozzi
    Pages 189-197

  16. Back Matter

    Pages 199-200
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Editors and Affiliations

  • Department of Physics, University of Padova, Padova, Italy

    Fabio Mammano

  • Facultad de Medicina, Universidad del Desarrollo, Santiago, Chile

    Mauricio Retamal

Bibliographic Information

  • Book Title: Connexin Hemichannels

  • Book Subtitle: Methods and Protocols

  • Editors: Fabio Mammano, Mauricio Retamal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3842-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3841-5Published: 06 April 2024

  • Softcover ISBN: 978-1-0716-3844-6Due: 10 May 2024

  • eBook ISBN: 978-1-0716-3842-2Published: 05 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 200

  • Number of Illustrations: 2 b/w illustrations, 38 illustrations in colour

  • Topics: Cell Biology

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