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Transmembrane β-Barrel Proteins

Methods and Protocols

  • Book
  • © 2024

Overview

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2778)

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About this book

This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane β-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Transmembrane β-Barrel Proteins: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of these vital membrane-spanning proteins.

Keywords

Table of contents (22 protocols)

Editors and Affiliations

  • Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, CNRS, Université de Toulouse, Toulouse, France

    Raffaele Ieva

Bibliographic Information

  • Book Title: Transmembrane β-Barrel Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Raffaele Ieva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3734-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3733-3Published: 14 March 2024

  • Softcover ISBN: 978-1-0716-3736-4Due: 14 April 2024

  • eBook ISBN: 978-1-0716-3734-0Published: 13 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 386

  • Number of Illustrations: 21 b/w illustrations, 54 illustrations in colour

  • Topics: Membrane Biology, Chemistry/Food Science, general

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