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Circular RNAs

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  • © 2024
  • Latest edition


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2765)

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About this book

This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to  gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on circular RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Circular RNAs, Second Edition aims to ensure successful results in the further study of this vital field.


Table of contents (17 protocols)

  1. circRNA Purification

  2. In Silico circRNA Profiling

  3. circRNA Detection, Sequence Validation and Quantification

  4. Functional Study: Tools for Loss- and Gain-of Function Analysis

  5. Functional Study: Biogenesis and Translation

Editors and Affiliations

  • Klaus Tschira Institute for Integrative Computational Cardiology, University Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  • Department of Cellular, Computational and Integrative Biology – CIBIO, University of Trento, Trento, Italy

    Marie-Laure Baudet

Bibliographic Information

  • Book Title: Circular RNAs

  • Editors: Christoph Dieterich, Marie-Laure Baudet

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3677-0Published: 22 February 2024

  • Softcover ISBN: 978-1-0716-3680-0Due: 24 March 2024

  • eBook ISBN: 978-1-0716-3678-7Published: 21 February 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 326

  • Number of Illustrations: 5 b/w illustrations, 53 illustrations in colour

  • Topics: Genetics and Genomics

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