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Bacteriophage Therapy

From Lab to Clinical Practice

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Joana Azeredo

    1. Centre of Biological Engineering, University of Minho, Braga, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Sanna Sillankorva

    1. International Iberian Nanotechnology Laboratory, Braga, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2734)

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About this book

This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls.

Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Isolation, and Characterization of Bacteriophages

    1. Front Matter

      Pages 1-1
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    2. Isolation of Bacteriophages for Clinically Relevant Bacteria

      • Sanna Sillankorva, Paul Hyman
      Pages 3-12

    3. Observation of Bacteriophage Ultrastructure by Cryo-Electron Microscopy

      • Ana Cuervo, Patricia Losana, José L. Carrascosa
      Pages 13-25

    4. Bacteriophage Taxonomy: A Continually Evolving Discipline

      • Dann Turner, Evelien M. Adriaenssens, Susan M. Lehman, Cristina Moraru, Andrew M. Kropinski
      Pages 27-45

  3. Selection, Production, and Encapsulation of Bacteriophages

    1. Front Matter

      Pages 47-47
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    2. Guidelines to Compose an Ideal Bacteriophage Cocktail

      • Maia Merabishvili, Jean-Paul Pirnay, Daniel De Vos
      Pages 49-66

    3. Rapid Bench to Bedside Therapeutic Bacteriophage Production

      • Tiffany Luong, Andrew D. Sue, Dwayne R. Roach
      Pages 67-88

    4. Bacteriophage Production in Compliance with Regulatory Requirements

      • Jean-Paul Pirnay, Maia Merabishvili, Daniel De Vos, Gilbert Verbeken
      Pages 89-115

    5. Nano/microformulations for Bacteriophage Delivery

      • Pilar Cortés, Mary Cano-Sarabia, Joan Colom, Jennifer Otero, Daniel Maspoch, Montserrat Llagostera
      Pages 117-130

  4. From In Vitro to Animal Efficacy Studies

    1. Front Matter

      Pages 131-131
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    2. Phage-Host Interaction Analysis Using Flow Cytometry

      • Maria Daniela Silva, Luís D. R. Melo
      Pages 133-140

    3. Bacteriophage Control of Infectious Biofilms

      • Luciana Meneses, Sanna Sillankorva, Joana Azeredo
      Pages 141-150

    4. Studying Bacteriophage Efficacy Using a Zebrafish Model

      • Marco Cafora, Alessia Brix, Francesca Forti, Federica Briani, Anna Pistocchi
      Pages 151-169

    5. Use of Galleria mellonella as an Animal Model for Studying the Antimicrobial Activity of Bacteriophages with Potential Use in Phage Therapy

      • Lucía Blasco, María Tomás
      Pages 171-180

  5. Host Interaction and Clinical Application

    1. Front Matter

      Pages 181-181
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    2. Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies

      • Katarzyna Gembara, Krystyna Dąbrowska
      Pages 183-196

    3. Bacteriophage Treatment of Infected Diabetic Foot Ulcers

      • Vera V. Morozova, Yulia N. Kozlova, Denis A. Ganichev, Nina V. Tikunova
      Pages 197-205

    4. A Review of Phage Therapy for Bone and Joint Infections

      • Tristan Ferry
      Pages 207-235

    5. Successful Use of Phage and Antibiotics Therapy for the Eradication of Two Bacterial Pathogens from the Respiratory Tract of an Infant

      • Vera V. Morozova, Yulia N. Kozlova, Nina V. Tikunova
      Pages 237-243
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Editors and Affiliations

  • Centre of Biological Engineering, University of Minho, Braga, Portugal

    Joana Azeredo

  • International Iberian Nanotechnology Laboratory, Braga, Portugal

    Sanna Sillankorva

Bibliographic Information

  • Book Title: Bacteriophage Therapy

  • Book Subtitle: From Lab to Clinical Practice

  • Editors: Joana Azeredo, Sanna Sillankorva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3523-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3522-3Published: 09 December 2023

  • Softcover ISBN: 978-1-0716-3525-4Due: 22 December 2024

  • eBook ISBN: 978-1-0716-3523-0Published: 08 December 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 321

  • Number of Illustrations: 3 b/w illustrations, 59 illustrations in colour

  • Topics: Virology, Microbiology, Behavioral Therapy

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