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The Olfactory System

  • Book
  • © 2023

Overview

Editors:
  1. Bradley J. Goldstein

    1. Department of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Hiroaki Matsunami

    1. Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2710)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Visualizing and Manipulating Olfactory Cilia Through Viral Delivery Coupled with En Face Imaging of Intact OE

    • Julien C. Habif, Chao Xie, Jeffrey R. Martens
    Pages 1-18

  3. Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa

    • Eman Abou Moussa, Melanie Makhlouf, Lisa S. Mathew, Luis R. Saraiva
    Pages 19-30

  4. Optical Activation of Photoswitchable TRPC Ligands in the Mammalian Olfactory System Using Laser Scanning Confocal Microscopy

    • Navin K. Ojha, Frank Zufall, Trese Leinders-Zufall
    Pages 31-47

  5. Intranasal Pressure Recording for Monitoring Mouse Respiration

    • Emma Janke, Janardhan P. Bhattarai, Minghong Ma
    Pages 49-60

  6. Identification of Immune Cells in Murine Olfactory Mucosa

    • Sebastian A. Wellford, E. Ashley Moseman
    Pages 61-69

  7. Chromatin Immunoprecipitation from Formaldehyde Cross-Linked Olfactory Sensory Neurons

    • Jerome K. Kahiapo, Kevin Monahan
    Pages 71-82

  8. Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence

    • Maira Harume Nagai, Hiroaki Matsunami
    Pages 83-97

  9. Measuring Cell Surface Expression of Odorant Receptors via Flow Cytometry

    • Jeevan Tewari, Hiroaki Matsunami
    Pages 99-109

  10. Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons

    • Qiang Wang, Tomoko Sengoku, William B. Titlow, Jennifer L. Strange, Timothy S. McClintock
    Pages 111-120

  11. Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis

    • Rhea Choi, Tiffany Ko, John B. Finlay, Ralph Abi Hachem, David Jang, Bradley J. Goldstein
    Pages 121-129

  12. Cranial Window for Acute and Chronic Optical Access to Record Neuronal Network Dynamics in the Olfactory Bulb

    • Marco Brondi, Claudia Lodovichi
    Pages 131-148

  13. Target-Captured mRNA from Murine Olfactory Bulb

    • Kevin W. Zhu, Hiroaki Matsunami
    Pages 149-170

  14. Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2

    • Ai Fang, Kaitlyn Petentler, Andrew Price, Seth Malloy, Michael Peterson, Lucinda Maddera et al.
    Pages 171-183

  15. Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration

    • Xiaochen Fu, Yu-Pei Huang, Sander Lindeman, Adam Mago, Izumi Fukunaga
    Pages 185-193

  16. Techniques in Staining of Rodent and Human Olfactory Tissue

    • Hironobu Nishijima, Eric H. Holbrook
    Pages 195-207

  17. Electrophysiological Recordings from Identified Cell Types in the Olfactory Cortex of Awake Mice

    • Kevin A. Bolding, Kevin M. Franks
    Pages 209-221

  18. Correction to: Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2

    • Ai Fang, Kaitlyn Petentler, Andrew Price, Seth Malloy, Michael Peterson, Lucinda Maddera et al.
    Pages C1-C1

  19. Back Matter

    Pages 223-224
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Keywords

About this book

This volume discusses the latest approaches used to investigate molecular biology and physiology in the peripheral and central olfactory system. Understanding both normal function and pathobiology provides readers with insight into understanding human olfactory disorders. The chapters in this book cover topics such as techniques for manipulating and measuring olfactory cilia function; approaches for investigating spatial RNA expression in olfactory mucosa; the use of chromatin immunoprecipitation with olfactory neurons; in vivo methods to label and identify activated olfactory neurons; defining organization in the olfactory bulbs and cortex; and the use of optical recording in the mouse olfactory bulb for acute or chronic experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The Olfactory System: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this important and developing field.



Editors and Affiliations

  • Department of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, USA

    Bradley J. Goldstein

  • Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, USA

    Hiroaki Matsunami

Bibliographic Information

  • Book Title: The Olfactory System

  • Editors: Bradley J. Goldstein, Hiroaki Matsunami

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3425-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3424-0Published: 10 September 2023

  • Softcover ISBN: 978-1-0716-3427-1Due: 12 November 2023

  • eBook ISBN: 978-1-0716-3425-7Published: 09 September 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 224

  • Number of Illustrations: 3 b/w illustrations, 43 illustrations in colour

  • Topics: Biomedicine, general

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