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Methods and Protocols

  • Book
  • © 2023
  • Latest edition


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2967)

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About this book

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 


Table of contents (19 protocols)

Editors and Affiliations

  • CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal

    Lucília Domingues

Bibliographic Information

  • Book Title: PCR

  • Book Subtitle: Methods and Protocols

  • Editors: Lucília Domingues

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3357-1Published: 23 August 2023

  • Softcover ISBN: 978-1-0716-3360-1Due: 23 September 2023

  • eBook ISBN: 978-1-0716-3358-8Published: 22 August 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 254

  • Number of Illustrations: 17 b/w illustrations, 30 illustrations in colour

  • Topics: Biotechnology

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