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Cell-Secreted Vesicles

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Seppo Vainio

    1. GeneCellNano, Oulu University, Oulu, Finland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2668)

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About this book

This detailed volume presents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as specific systems and models allowing for the study of EVs of different origin. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell-Secreted Vesicles: Methods and Protocols serves as an ideal guide to conducting systematic assays in an effort to further our understanding of the mode of assembly, secretion, and targeting of EVs which will serve eventually as new therapeutic openings.

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Purification and Analysis of Single or Multiple EVs

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Plasmon-Enhanced Characterization of Single Extracellular Vesicles

      • Mi Ho Jeong, Taehwang Son, Hyungsoon Im
      Pages 3-13

    3. Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles

      • Yang Li, Yunpeng Wang, Jinwei Tian, Jian-An Huang
      Pages 15-22

    4. In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein

      • Gloria I. Perez, Michael H. Bachmann, Masamitsu Kanada
      Pages 23-32

    5. Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy

      • Maribel Lara Corona, Ilse Hurbain, Graça Raposo, Guillaume van Niel
      Pages 33-43

    6. Extracellular Vesicle Isolation by a Tangential-Flow Filtration-Based Large-Scale Purification Method

      • Runjie Yuan, Yijun Zhou, Gabriel F. Arias, Dirk P. Dittmer
      Pages 45-55

    7. Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography–Mass Spectrometry

      • Marta Martin-Lorenzo, Dolores Molero, Gloria Alvarez-Llamas
      Pages 57-68

    8. Methodologies for Scalable Production of High-Quality Purified Small Extracellular Vesicles from Conditioned Medium

      • Gabriella Andriolo, Elena Provasi, Andrea Brambilla, Stefano Panella, Sabrina Soncin, Viviana Lo Cicero et al.
      Pages 69-98

    9. Automated On-Line Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles

      • Susanne K. Wiedmer, Evgen Multia, Thanaporn Liangsupree, Marja-Liisa Riekkola
      Pages 99-108

  3. Targeting Cell Behavior Functions of EVs

    1. Front Matter

      Pages 109-109
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    2. A Novel Assay for Investigating the Role of Exosomes in Tumor Cell-Endothelial Cell Crosstalk

      • Yan Qiu, Wenli Jiang, Ye Zeng
      Pages 111-119

    3. Nanoparticle (NP) Loading by Direct Incubation with Extracellular Vesicles-Secretor Cells: NP Encapsulation and Exosome Characterization

      • Ana Martín-Pardillos, Pilar Martín-Duque
      Pages 121-132

    4. Two Complementary Strategies to Quantitate Extracellular Vesicle Uptake Using Bioluminescence and Non-Lipidic Dyes

      • Víctor Toribio, María Yáñez-Mó
      Pages 133-144

    5. Integrin-Mediated Exosomal Homing to Organs

      • Eun Jeong Park, Motomu Shimaoka
      Pages 145-158

    6. Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research

      • Manjusha Vaidya, Nasif Sayeed, Caroline Hobson, Sandeep Sreerama, Jonhoi Smith, Riya Shah et al.
      Pages 159-189

    7. Quantitative Analysis of Extracellular Vesicle Release Using Artificial MicroRNAs

      • Albert Lu
      Pages 191-207

  4. Systems and Models to Study EVs

    1. Front Matter

      Pages 209-209
      Download chapter PDF

    2. Purification of Bacterial-Enriched Extracellular Vesicle Samples from Feces by Density Gradient Ultracentrifugation

      • Nadiya Byts, Olha Makieieva, Artem Zhyvolozhnyi, Genevieve Bart, Johanna Korvala, Jenni Hekkala et al.
      Pages 211-226

    3. Isolation and Characterization of Extracellular Vesicles from Lymphocytes

      • Lujain Al-Ghadir, Zhi Chen
      Pages 227-239
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Editors and Affiliations

  • GeneCellNano, Oulu University, Oulu, Finland

    Seppo Vainio

Bibliographic Information

  • Book Title: Cell-Secreted Vesicles

  • Book Subtitle: Methods and Protocols

  • Editors: Seppo Vainio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3203-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3202-4Published: 05 May 2023

  • Softcover ISBN: 978-1-0716-3205-5Due: 05 June 2023

  • eBook ISBN: 978-1-0716-3203-1Published: 04 May 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 315

  • Number of Illustrations: 20 b/w illustrations, 40 illustrations in colour

  • Topics: Cell Biology, Biomechanics

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