Plant Iron Homeostasis

1. Front Matter

   Pages i-xi

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2. High-Throughput Plant Gene Expression Analysis by 384-Format Reverse Transcription-Quantitative PCR for Investigating Plant Iron Homeostasis

   • Mary Njeri Ngigi, Petra Bauer

   Pages 1-22

3. The Use of Spectral Imaging to Follow the Iron and pH-Dependent Accumulation of Fluorescent Coumarins

   • Kevin Robe, Geneviève Conjero, Christian Dubos

   Pages 23-30

4. Assay of Fe(III) Chelate Reductase Activity in Arabidopsis thaliana Root

   • Sun A. Kim

   Pages 31-36

5. An Adapted Protocol for Quantitative Rhizosphere Acidification Assay

   • Sara Omer, Claire Macero, Dayishaa Daga, Kelly Zheng, Jeeyon Jeong

   Pages 37-46

6. Techniques to Study Common Root Responses to Beneficial Microbes and Iron Deficiency

   • Shu-Hua Hsu, Max J. J. Stassen, Corné M. J. Pieterse, Ioannis A. Stringlis

   Pages 47-62

7. Imaging and Quantifying the Endocytosis of IRON-REGULATED TRANSPORTER1 from Arabidopsis

   • Julien Spielmann, Julie Neveu, Grégory Vert

   Pages 63-73

8. Label-Free Quantitative Proteomics in Plant

   • Ruonan Wang, Peijun Zhou, Yilin Pan, Lu Zheng, Xiaoying Dong, Renfang Shen et al.

   Pages 75-83

9. Chromatin Immunoprecipitation (ChIP) to Study the Transcriptional Regulatory Network that Controls Iron Homeostasis in Arabidopsis thaliana

   • Fei Gao, Christian Dubos

   Pages 85-94

10. Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications

    • Yang Yu, Yuxin Wang, Zhujun Yao, Ziqin Wang, Zijun Xia, Joohyun Lee

    Pages 95-111

11. Arabidopsis Micro-grafting to Study the Systemic Signaling of Nutrient Status

    • En-Jung Hsieh, Louis Grillet

    Pages 113-120

12. Advances in Iron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms

    • Maria A. Pagani, Diego F. Gomez-Casati

    Pages 121-145

13. Functional Analysis of Chloroplast Iron Uptake and Homeostasis

    • Helga Zelenyánszki, Ádám Solti

    Pages 147-171

14. Perls/DAB Staining to Examine Iron Distribution in Arabidopsis Embryos

    • Joaquín Vargas, Hannetz Roschzttardtz

    Pages 173-176

15. Visualizing Metal Distribution in Plants Using Synchrotron X-Ray Fluorescence Microscopy Techniques

    • Ju-Chen Chia, Arthur R. Woll, Louisa Smieska, Olena K. Vatamaniuk

    Pages 177-189

16. A Simple Semi-hydroponic System for Studying Iron Homeostasis in Maize

    • Stavroula Fili, Elsbeth Walker

    Pages 191-201

17. Optimizing Fe Nutrition for Algal Growth

    • Anne G. Glaesener, Sabeeha S. Merchant

    Pages 203-215

18. Back Matter

    Pages 217-218

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This detailed volume focuses on iron homeostasis in plants, iron being an essential micronutrient that serves as a cofactor in numerous metabolic processes but is harmful in excess. Specifically, the content ranges from protocols to study the iron deficiency response, the interaction between root and microbes under iron deficient conditions, the transcriptional network of iron homeostasis, systemic signaling of iron, chloroplast iron regulation, as well as methods on quantitative proteomics, histochemical iron staining, metal imaging using x-ray fluorescence microscopy, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Iron Homeostasis: Methods and Protocols serves as a valuable resource for the plant iron homeostasis research community and will be of broad interest to plant biologists, soil scientists, and molecular biologists.

• Plant growth and development
• Iron deficiency response
• Iron-induced toxicity
• Systemic signaling
• Crop plants

• Department of Biology, Amherst College, Amherst, USA

  Jeeyon Jeong