Editors:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2633)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, access via your institution.
Table of contents (17 protocols)
-
Front Matter
-
Back Matter
About this book
This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.
Keywords
- plasmid cloning
- Site specific Recombinases
- DNA Aptamers
- CRispR-Cas9
- Xenopus
- cDNA Libraries
- Capillary electrophoresis
Editors and Affiliations
-
Biophysics Laboratories, School of Biological Sciences, University of Portsmouth, Portsmouth, UK
Garry Scarlett
Bibliographic Information
Book Title: DNA Manipulation and Analysis
Editors: Garry Scarlett
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3004-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-3003-7Published: 01 March 2023
Softcover ISBN: 978-1-0716-3006-8Due: 15 March 2024
eBook ISBN: 978-1-0716-3004-4Published: 28 February 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 249
Number of Illustrations: 5 b/w illustrations, 44 illustrations in colour
Topics: Genetics and Genomics