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MicroRNA Detection and Target Identification

Methods and Protocols

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  • © 2023
  • Latest edition


  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2630)

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About this book

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

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Table of contents (16 protocols)

Editors and Affiliations

  • RNA Biology, University of East Anglia, Norwich, UK

    Tamas Dalmay

Bibliographic Information

  • Book Title: MicroRNA Detection and Target Identification

  • Book Subtitle: Methods and Protocols

  • Editors: Tamas Dalmay

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2981-9Published: 24 January 2023

  • Softcover ISBN: 978-1-0716-2984-0Published: 24 January 2024

  • eBook ISBN: 978-1-0716-2982-6Published: 23 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 266

  • Number of Illustrations: 5 b/w illustrations, 36 illustrations in colour

  • Topics: Genetics and Genomics, Biochemistry, general

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