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Virus-Host Interactions

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2610)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Genetic Tools for Studying the Roles of Sphingolipids in Viral Infections

    • Gaelen Guzman, Cameron Creek, Scotland Farley, Fikadu G. Tafesse
    Pages 1-16
  3. Detection of Serum Antibody Responses in Nipah Virus–Infected Pigs

    • Kerstin Fischer, Bradley Pickering, Sandra Diederich
    Pages 17-29
  4. Computational Integration of HSV-1 Multi-omics Data

    • Caroline C. Friedel
    Pages 31-48
  5. A Cell Membrane Fusion Assay for the Fish Pathogen Spring Viremia of Carp Virus (SVCV)

    • Ainhoa Gómez-Quintanilla, David Talavera, Luis Perez
    Pages 49-56
  6. Viral Quantification in Bee Samples Using Synthetic DNA Sequences with Real-Time PCR (qPCR)

    • Nuria Morfin, Jose O. Macías-Macías, Ernesto Guzman-Novoa
    Pages 57-66
  7. Computational Modeling of IN-CTD/TAR Complex to Elucidate Additional Strategies to Inhibit HIV-1 Replication

    • Liming Qiu, Savita Bhutoria, Ganjam V. Kalpana, Xiaoqin Zou
    Pages 75-84
  8. 4C Analysis of EBV-Host DNA Interactome

    • Kyoung-Dong Kim, Paul M. Lieberman
    Pages 99-107
  9. Detection Methods for H1N1 Virus

    • Ravina, Subodh, Krishna Kant Sharma, Hari Mohan
    Pages 109-127
  10. Detection of SARS-CoV-2 in Human Breast Milk

    • Rüdiger Groß, Carina Conzelmann, Jan Münch, Janis A. Müller
    Pages 129-135
  11. The Rhizopus Holobiont: A Model to Decipher Fungal–Bacterial–Viral Symbioses

    • Astrid N. Espino-Vázquez, Gonzalo Córdova-López, J. Francisco Cabrera-Rangel, J. Valeria Mendoza-Servín, Laila P. Partida-Martínez
    Pages 137-147
  12. Three-Dimensional Human Brain Organoids to Model HIV-1 Neuropathogenesis

    • Roberta S. dos Reis, Shilpa Sant, Velpandi Ayyavoo
    Pages 167-178
  13. A Lentiviral Pseudotype System to Characterize SARS-CoV-2 Glycoprotein

    • Dan Mi, Jiaxin Hu, Zhaohui Qian
    Pages 187-199
  14. Back Matter

    Pages 201-202

About this book

This detailed volume spotlights methods to investigate a variety of virus-host interactions in humans, other mammals, fish, or insects. It explores viruses such as white spot syndrome virus (WSSV), honeybee viruses, Nipah virus, EBV, SVCV, HSV-1, HIV-1, A H1N1, and SARS-CoV-2, as well as applications of techniques such as qPCR, serum antibody responses, 4C analysis, cell membrane fusion, biosensors, computational modelling, quantitative proteomics, and other genetic tools to decipher those viral infections and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Virus-Host Interactions: Methods and Protocols serves as a valuable resource for researchers both in academia and in the biosciences industry who are engaged in the search for a better understanding of threatening virus-hosts interactions, virus detection, their characterization, and ultimately their taming and control.

Keywords

  • Host cells
  • Viral infections
  • Infection mechanisms
  • Serum antibody responses
  • Animal models
  • Computational modeling

Editors and Affiliations

  • Center for Radiological Research, Columbia University Irving Medical Cente, New York, USA

    Marilena Aquino de Muro

Bibliographic Information

  • Book Title: Virus-Host Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Marilena Aquino de Muro

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2895-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2894-2Published: 20 December 2022

  • Softcover ISBN: 978-1-0716-2897-3Due: 03 January 2024

  • eBook ISBN: 978-1-0716-2895-9Published: 19 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 202

  • Number of Illustrations: 13 b/w illustrations, 31 illustrations in colour

  • Topics: Virology, Biological Techniques

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
This title has not yet been released. You may pre-order it now and we will ship your order when it is published on 3 Jan 2024.
  • Compact, lightweight edition
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access