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Poly(ADP-Ribose) Polymerase

Methods and Protocols

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  • © 2023
  • Latest edition


  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2609)

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Table of contents (26 protocols)

  1. Poly(ADP-Ribosyl)ating Pathway in Chromatin and Gene Expression

  2. Using Animal Models

  3. Designing and Testing PARP-1 Inhibitors

  4. Targeting the Understudied Components of pADPr Pathway


About this book

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.

Editors and Affiliations

  • Department of Biomedical Sciences, University of North Dakota, Grand Forks, USA

    Alexei V. Tulin

Bibliographic Information

  • Book Title: Poly(ADP-Ribose) Polymerase

  • Book Subtitle: Methods and Protocols

  • Editors: Alexei V. Tulin

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2890-4Published: 15 December 2022

  • Softcover ISBN: 978-1-0716-2893-5Published: 15 December 2023

  • eBook ISBN: 978-1-0716-2891-1Published: 14 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 446

  • Number of Illustrations: 37 b/w illustrations, 61 illustrations in colour

  • Topics: Cell Biology, Chemistry/Food Science, general, Enzymology

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