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Cell Migration in Three Dimensions

  • Book
  • © 2023


  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2608)

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Table of contents (25 protocols)

  1. The Cell Biology of Cell Migration

  2. Developmental Model Systems to Assess Cell Migration During Morphogenesis

  3. Cell Migration in Cancers and the Tumor Microenvironment


About this book

This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes. 

Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via

Editors and Affiliations

  • Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam University Medical Center, Amsterdam, The Netherlands

    Coert Margadant

Bibliographic Information

  • Book Title: Cell Migration in Three Dimensions

  • Editors: Coert Margadant

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2886-7Published: 19 January 2023

  • Softcover ISBN: 978-1-0716-2889-8Published: 19 January 2024

  • eBook ISBN: 978-1-0716-2887-4Published: 18 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 472

  • Number of Illustrations: 18 b/w illustrations, 102 illustrations in colour

  • Topics: Cell Biology, Cell Biology

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