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The Ubiquitin Code

  • Book
  • © 2023


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2602)

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About this book

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field. 

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Table of contents (17 protocols)

  1. Chain Diversity

  2. Ub and UbL Chemical Tools

  3. Methods to Study Ubiquitin Conjugating Enzymes (E3s)

  4. Methods to Study DUBs

  5. Methods to Identify New Targets and Regulators

Editors and Affiliations

  • LCC-UPR 8241-CNRS Equipe M, Laboratoire de Chimie de Coordination, Toulouse, France

    Manuel S Rodriguez

  • CIC bioGUNE, Derio, Spain

    Rosa Barrio

Bibliographic Information

  • Book Title: The Ubiquitin Code

  • Editors: Manuel S Rodriguez, Rosa Barrio

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2858-4Published: 30 November 2022

  • Softcover ISBN: 978-1-0716-2861-4Published: 30 November 2023

  • eBook ISBN: 978-1-0716-2859-1Published: 29 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 245

  • Number of Illustrations: 9 b/w illustrations, 36 illustrations in colour

  • Topics: Chemistry/Food Science, general, Cell Biology

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