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Methods and Protocols

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  • © 2023
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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2555)

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About this book

This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.

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Table of contents (17 protocols)

Editors and Affiliations

  • Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany

    Wolfgang R. Streit

  • Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany

    Rolf Daniel

Bibliographic Information

  • Book Title: Metagenomics

  • Book Subtitle: Methods and Protocols

  • Editors: Wolfgang R. Streit, Rolf Daniel

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2794-5Published: 29 October 2022

  • Softcover ISBN: 978-1-0716-2797-6Published: 29 October 2023

  • eBook ISBN: 978-1-0716-2795-2Published: 28 October 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 287

  • Number of Illustrations: 8 b/w illustrations, 34 illustrations in colour

  • Topics: Genetics and Genomics, Microbial Genetics and Genomics, Enzymology

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