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Plant Pathology

Method and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2536)

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-2517-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
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  • Tax calculation will be finalised during checkout
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xviii
  2. Plant Disease Diagnosis: Traditional Methods

    1. Front Matter

      Pages 1-1
    2. Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers

      • Carla S. Pimentel, Paulo N. Firmino, Matthew P. Ayres
      Pages 3-11
    3. Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis

      • Cristina Zamora-Ballesteros, Reinaldo Pire, Julio Javier Diez
      Pages 51-73
  3. DNA Extraction Protocols

    1. Front Matter

      Pages 75-75
    2. DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues

      • Domenico Rizzo, Daniele Da Lio, Linda Bartolini, Cristina Francia, Antonio Aronadio, Nicola Luchi et al.
      Pages 91-101
    3. A Rapid Method for Extracting High-Quality DNA from Soils

      • Anna Maria Vettraino, Nicola Luchi
      Pages 103-107
  4. Molecular Methods to Detect Plant Pathogens

    1. Front Matter

      Pages 109-109
    2. Invasive Alien Plant Pathogens: The Need of New Detection Methods

      • Alberto Santini, Duccio Migliorini
      Pages 111-118
    3. Detection and Identification of the Causal Agents of Dothistroma Needle Blight

      • Funda Oskay, Asko Lehtijärvi, Hatice Tuğba Doğmuş Lehtijärvi
      Pages 155-166
    4. Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors

      • Karolina Pusz-Bochenska, Edel Pérez-López, Tim J. Dumonceaux, Chrystel Olivier, Tyler J. Wist
      Pages 179-199
    5. Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR

      • Ricardo Delgado Santander, Katarina Gašić, Srđan Goran Aćimović
      Pages 231-249

About this book

This volume covers the latest developments in different areas of plant pathology. The chapters in this volume are organized into seven parts. Part One provides traditional methods for isolation and identification of invasive pathogens and root disease. Part Two looks at new and rapid DNA extraction protocols from different samples, and Part Three focuses on molecular detection protocols for identifying and quantifying plant pathogens, including fungal and bacterial invasive species. Part Four describes the application of metabarcoding in plant pathology, and Part Five talks about plant pathogen interactions. Part Six concentrates on population genomics of plant pathogens, and Part Seven covers biocontrol on plant pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Pathology: Methods and Protocols is a valuable resource for researchers in the plant pathology community, and discusses various approaches for the detection, identification, and control of plant diseases.


Keywords

  • Heterobasidion
  • Magnetic Ionic Liquids
  • Xylellafastidiosa
  • qPCR
  • HTS

Editors and Affiliations

  • Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Sesto Fiorentino, Florence, Italy

    Nicola Luchi

Bibliographic Information

  • Book Title: Plant Pathology

  • Book Subtitle: Method and Protocols

  • Editors: Nicola Luchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2517-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2516-3Published: 13 July 2022

  • Softcover ISBN: 978-1-0716-2519-4Due: 27 July 2023

  • eBook ISBN: 978-1-0716-2517-0Published: 12 July 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 497

  • Number of Illustrations: 10 b/w illustrations, 96 illustrations in colour

  • Topics: Plant Science, Plant Pathology

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-2517-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 219.99
Price excludes VAT (USA)