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  • © 2022

Candida auris

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2517)

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-2417-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
  2. Isolation and Identification of Candida auris

    1. Front Matter

      Pages 1-1
    2. Isolation of Candida auris in Clinical Specimens

      • Anamika Yadav, Ashutosh Singh, Anuradha Chowdhary
      Pages 3-20
    3. Candida auris Identification and Profiling by MALDI–ToF Mass Spectrometry

      • Alireza Abdolrasouli, Mark A. Fraser
      Pages 21-32
    4. Molecular Tools for Candida auris Identification and Typing

      • Auke W. de Jong, Bert Gerrits van den Ende, Ferry Hagen
      Pages 33-41
    5. qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec–qPCR Test

      • Antonio Martínez-Murcia, Gema Bru, Aaron Navarro
      Pages 43-51
  3. Testing Susceptibility of Candida auris to Antifungal Drugs

    1. Front Matter

      Pages 53-53
    2. Susceptibility Testing of Candida auris Isolates

      • Elizabeth M. Johnson, Andrew M. Borman
      Pages 55-71
  4. Genetic Manipulation of Candida auris

    1. Front Matter

      Pages 87-87
    2. An Optimized Electroporation Procedure for Genetic Transformation of Candida auris

      • Dalal Kasir, Marwan Osman, Monzer Hamze, Jean-Philippe Bouchara, Vincent Courdavault, Nicolas Papon
      Pages 89-94
    3. Downregulation of Essential Genes in the Fungal Pathogen Candida auris

      • Kali R. Iyer, Sang Hu Kim, Nicole Robbins, Leah E. Cowen
      Pages 111-126
  5. Phenotypic and Biochemical Characterization of Candida auris

    1. Front Matter

      Pages 127-127
    2. Cell Viability Assays for Candida auris

      • Jason L. Brown, Bryn Short, Alicia Ware, Leighann Sherry, Ryan Kean, Gordon Ramage
      Pages 129-153
    3. Measuring Gene Expression via mRNA Abundance in Candida auris

      • Mohd Wasi, Remya Nair, Nitesh Kumar Khandelwal, Rajendra Prasad
      Pages 155-164
    4. Gas Chromatography–Mass Spectrometry (GC–MS) Analysis of Candida auris Metabolites

      • Bahgat Fayed, Ghalia Kohder, Sameh S. M. Soliman
      Pages 165-172
    5. Isolation of Extracellular Vesicles from Candida auris

      • Daniel Zamith-Miranda, Lysangela R. Alves, Marcio L. Rodrigues, Leonardo Nimrichter, Joshua D. Nosanchuk
      Pages 173-178

About this book

This volume covers the latest experimental protocols used by researchers to study the recently emerged fungal pathogen of humans, Candida auris, and also explores the pathogen’s antifungal resistance, virulence, and pathogenicity. The chapters in this book cover topics such as the identification of C. auris in clinic samples by PCR and mass spectrometry; molecular typing to identify clade affiliation; genetic manipulation; genome analysis by bioinformatics; and phenotypic characterization using various biochemical, mass spectrometry, microscopy, and flow cytometry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Candida auris: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field.


Keywords

  • MALDI-TOF MS
  • Echinocandins
  • Cell viability assays
  • Hsp90
  • Fungal Predators

Editors and Affiliations

  • Institute of Medical Sciences (IMS), University of Aberdeen, Aberdeen, UK

    Alexander Lorenz

Bibliographic Information

  • Book Title: Candida auris

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander Lorenz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2417-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2416-6Published: 09 June 2022

  • Softcover ISBN: 978-1-0716-2419-7Due: 23 June 2023

  • eBook ISBN: 978-1-0716-2417-3Published: 08 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 342

  • Number of Illustrations: 16 b/w illustrations, 60 illustrations in colour

  • Topics: Microbiology, Fungi

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-2417-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 219.99
Price excludes VAT (USA)