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Extracellular Vesicles in Diagnosis and Therapy

  • Book
  • © 2022

Overview

Editors:
  1. Maurizio Federico

    1. National Center for Global Health, Istituto Superiore di Sanità, Rome, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Barbara Ridolfi

    1. National Center for Global Health, Istituto Superiore di Sanità, Rome, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2504)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Extracellular Vesicles Detection and Isolation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Aptasensors for Cancerous Exosome Detection

      • Jin Li, Sitao Xie, Fengli Qu, Weihong Tan
      Pages 3-20

    3. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

      • Lizhou Xu, Khuloud T. Al-Jamal
      Pages 21-30

    4. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

      • Kaloyan Takov, I-Jou Teng, Manuel Mayr
      Pages 31-40

    5. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis

      • Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, Liam A. McDonnell
      Pages 41-54

    6. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry

      • Najat Hajji, Chi M. Hau, Rienk Nieuwland, Edwin van der Pol
      Pages 55-75

  3. Isolation and Characterization of Tissue and Biofluid-Specific EVs

    1. Front Matter

      Pages 77-77
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    2. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles

      • Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, Carlos Salomon
      Pages 79-89

    3. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells

      • Qiang Wu, Min-Xia Ke, Huang-Tian Yang
      Pages 91-100

    4. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery

      • Lucas Trevisan França de Lima, Juliana Müller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, Chamindie Punyadeera
      Pages 101-112

    5. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance

      • Marlene Reithmair, Anja Lindemann, Veronika Mussack, Michael W. Pfaffl
      Pages 113-133

  4. Analysis of EV Components

    1. Front Matter

      Pages 135-135
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    2. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay

      • Ayyanar Sivanantham, Heedoo Lee, Yang Jin
      Pages 137-145

    3. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles

      • Anton B. Iliuk
      Pages 147-156

    4. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum

      • Yuchen Sun, Kosuke Saito, Yoshiro Saito
      Pages 157-173

  5. EV Engineering

    1. Front Matter

      Pages 175-175
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    2. Extracellular Vesicles and Their Use as Vehicles of Immunogens

      • Chiara Chiozzini, Barbara Ridolfi, Maurizio Federico
      Pages 177-198

    3. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells

      • Noelle Leary, Sarina Walser, Lothar C. Dieterich
      Pages 199-206

    4. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles

      • Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, Francesco Manfredi
      Pages 207-217
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Keywords

About this book

This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.

Editors and Affiliations

  • National Center for Global Health, Istituto Superiore di Sanità, Rome, Italy

    Maurizio Federico, Barbara Ridolfi

Bibliographic Information

  • Book Title: Extracellular Vesicles in Diagnosis and Therapy

  • Editors: Maurizio Federico, Barbara Ridolfi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2341-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2340-4Published: 26 April 2022

  • Softcover ISBN: 978-1-0716-2343-5Published: 26 April 2023

  • eBook ISBN: 978-1-0716-2341-1Published: 25 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 243

  • Number of Illustrations: 14 b/w illustrations, 31 illustrations in colour

  • Topics: Membrane Biology, Biomedicine, general

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