Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2492)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Similar content being viewed by others
Keywords
Table of contents (20 protocols)
-
Front Matter
-
Introduction to Blood-Brain Barrier (BBB) Physiology
-
Front Matter
-
-
Using Pluripotent Stem Cells in Models of the BBB
-
Front Matter
-
-
Co-culture, Permeability, and Transwell Models of the BBB: Methods for Studying Shear Stress, Barrier Integrity and Breakdown
-
Front Matter
-
-
Microfluidic Chip Models of the BBB
-
Front Matter
-
-
Models to Study Specific Pathologies at the BBB: Cancer, Neurodegeneration, and More
-
Front Matter
-
Editors and Affiliations
Bibliographic Information
Book Title: The Blood-Brain Barrier
Book Subtitle: Methods and Protocols
Editors: Nicole Stone
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2289-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2288-9Published: 24 June 2022
Softcover ISBN: 978-1-0716-2291-9Published: 25 June 2023
eBook ISBN: 978-1-0716-2289-6Published: 22 June 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 357
Number of Illustrations: 10 b/w illustrations, 73 illustrations in colour
Topics: Anatomy, Neurosciences