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TGF-Beta Signaling

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Zhike Zi

    1. Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment, Berlin, Germany

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  2. Xuedong Liu

    1. Department of Biochemistry, University of Colorado, Boulder, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2488)

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About this book

This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-β) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-β signaling studies, with others exploring the increasing role of both modeling and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-β field into the quantitative realm.

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot

    • Difan Deng, Zhike Zi
    Pages 1-12

  3. Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter

    • Adilson Fonseca Teixeira, Josie Iaria, Hong-Jian Zhu
    Pages 13-22

  4. Complex Formation Among TGF-β Receptors in Live Cell Membranes Measured by Patch-FRAP

    • Szabina Szófia Szilágyi, Orit Gutman, Yoav I. Henis
    Pages 23-34

  5. Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein–Protein Proximity

    • Jianying Yang
    Pages 35-45

  6. Visualizing Dynamic Changes During TGF-β-Induced Epithelial to Mesenchymal Transition

    • Abhishek Sinha, Pranav Mehta, Chuannan Fan, Jing Zhang, Dieuwke L. Marvin, Maarten van Dinther et al.
    Pages 47-65

  7. Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-β Family Signaling in Human Breast Cancer Progression

    • Chao Li, Jin Ma, Arwin Groenewoud, Jiang Ren, Sijia Liu, B. Ewa Snaar-Jagalska et al.
    Pages 67-80

  8. Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling

    • Zixin Huang, Alexander Loewer
    Pages 81-97

  9. CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators

    • Nina Frey, Gerald Schwank
    Pages 99-111

  10. Optogenetic Control of TGF-β Signaling

    • Yuchao Li, Zhike Zi
    Pages 113-124

  11. Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells

    • Fabien Furfaro, Carine Vias, Benoit Sorre
    Pages 125-143

  12. Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network

    • Leijun Ye, Chunhe Li
    Pages 145-157

  13. Discrete Logic Modeling of Cell Signaling Pathways

    • Nensi Ikonomi, Silke D. Werle, Julian D. Schwab, Hans A. Kestler
    Pages 159-181

  14. Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering

    • Maciej Dobrzyński, Marc-Antoine Jacques, Olivier Pertz
    Pages 183-206

  15. Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst

    • Marja Kornhuber, Sebastian Dunst
    Pages 207-226

  16. Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs

    • Zeyu Liu, Adrian Ramirez, Xuedong Liu
    Pages 227-236

  17. Back Matter

    Pages 237-239
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Editors and Affiliations

  • Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment, Berlin, Germany

    Zhike Zi

  • Department of Biochemistry, University of Colorado, Boulder, USA

    Xuedong Liu

Bibliographic Information

  • Book Title: TGF-Beta Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Zhike Zi, Xuedong Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2277-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2276-6Published: 29 March 2022

  • Softcover ISBN: 978-1-0716-2279-7Published: 29 March 2022

  • eBook ISBN: 978-1-0716-2277-3Published: 28 March 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 239

  • Number of Illustrations: 4 b/w illustrations, 45 illustrations in colour

  • Topics: Biomechanics, Cell Biology

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