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Plant Proteases and Plant Cell Death

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2447)

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eBook USD 149.00
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  • ISBN: 978-1-0716-2079-3
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  • Tax calculation will be finalised during checkout
Hardcover Book USD 199.99
Price excludes VAT (USA)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I

    • Vida Štrancar, Katarina Petra van Midden, Marina Klemenčič, Christiane Funk
    Pages 1-11
  3. Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii

    • Igor Sabljić, Yong Zou, Marina Klemenčič, Christiane Funk, Jerry Ståhlberg, Peter Bozhkov
    Pages 13-20
  4. Expression in Escherichia coli, Refolding, and Purification of Plant Aspartic Proteases

    • Pedro Castanheira, Carla Almeida, Daniela Dias-Pedroso, Isaura Simões
    Pages 21-33
  5. Purification of His-Tagged Proteases from the Apoplast of Agroinfiltrated N. benthamiana

    • Mariana Schuster, Judith K. Paulus, Jiorgos Kourelis, Renier A. L. van der Hoorn
    Pages 53-66
  6. Identification of Cognate Protease/Substrate Pairs by Use of Class-Specific Inhibitors

    • Annick Stintzi, Nils Stührwohldt, Stefanie Royek, Andreas Schaller
    Pages 67-81
  7. Detection of Apoplastic Protease Inhibitorsinhibitors Using Convolution Activity-Based Protein Profiling

    • Andrea Passarge, Gunther Doehlemann, Johana C. Misas Villamil
    Pages 95-104
  8. Cysteine Reactivity Profiling to Unveil Redox Regulation in Phytopathogens

    • Kyoko Morimoto, Monika Stegmann, Farnusch Kaschani, Shabaz Mohammed, Renier A. L. van der Hoorn
    Pages 105-117
  9. Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants

    • Simon Stael, Luke P. Miller, Álvaro D. Fernández-Fernández, Frank Van Breusegem
    Pages 127-137
  10. Sensitive Plant N-Terminome Profiling with HUNTER

    • Fatih Demir, Andreas Perrar, Melissa Mantz, Pitter F. Huesgen
    Pages 139-158
  11. Profiling Sequence Specificity of Proteolytic Activities Using Proteome-Derived Peptide Libraries

    • Fatih Demir, Maithreyan Kuppusamy, Andreas Perrar, Pitter F. Huesgen
    Pages 159-174
  12. Monitoring Cell Death Via Ion Leakage and PAM Fluorometry

    • Nick Dunken, Lisa Mahdi, Rainer E. Häusler, Alga Zuccaro
    Pages 175-183
  13. Measuring and Perturbing Ferroptosis in Plants

    • Ayelen M. Distéfano, Fernanda Marchetti, Eduardo Zabaleta, Gabriela C. Pagnussat
    Pages 185-192
  14. Detection and Quantification of the Hypersensitive Response Cell Death in Arabidopsis thaliana

    • Jose Salguero-Linares, Saul Lema-Asqui, Marta Salas-Gómez, Andrea Froilán-Soares, Núria S. Coll
    Pages 193-204

About this book

This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Keywords

  • protease expression
  • CRISPR
  • HUNTER
  • PTM database
  • chlorophyll fluorescence

Editors and Affiliations

  • Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia

    Marina Klemenčič

  • VIB-UGent Cntr for Plant Systems Biology, Ghent University, Ghent, Belgium

    Simon Stael

  • ZEA-3 Analytik, Forschungszentrum Juelich, Juelich, Germany

    Pitter F. Huesgen

Bibliographic Information

  • Book Title: Plant Proteases and Plant Cell Death

  • Book Subtitle: Methods and Protocols

  • Editors: Marina Klemenčič, Simon Stael, Pitter F. Huesgen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2079-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2078-6

  • eBook ISBN: 978-1-0716-2079-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 299

  • Number of Illustrations: 5 b/w illustrations, 53 illustrations in colour

  • Topics: Plant Science

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-2079-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book USD 199.99
Price excludes VAT (USA)